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Warangalone

Catalog No. TQ0072   CAS 4449-55-2
Synonyms: Scandenolone

Warangalone is an anti-malarial compound that can inhibit the growth of both strains of parasite 3D7 (chloroquine-sensitive) and K1 (chloroquine-resistant) with IC50s of 4.8 μg/mL and 3.7 μg/mL, respectively.

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Warangalone Chemical Structure
Warangalone, CAS 4449-55-2
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Warangalone is an anti-malarial compound that can inhibit the growth of both strains of parasite 3D7 (chloroquine-sensitive) and K1 (chloroquine-resistant) with IC50s of 4.8 μg/mL and 3.7 μg/mL, respectively.
Targets&IC50 parasite 3D7:.8 μg/mL, cAK:3.5 μM, parasite K1:3.7 μg/mL
In vitro Warangalone can also inhibit cyclic AMP-dependent protein kinase catalytic subunit (cAK) with an IC50 of 3.5 μM [2]. When HL-60 cells are exposed to Warangalone (30 μM) for 24 h, Warangalone induces a significant decrease (8%) in cell viability compared to controls. Warangalone also inhibits HL-60 cell growth within 24 h in a time-dependent fashion. A time-dependent increase in caspase-9 activity is observed in Warangalone-treated cells [3].
Kinase Assay Cells are seeded in 24-well plates at a density of 3×10^6 cells per well. After exposure of the cells to Warangalone for the allotted time periods, the cells are washed three times with PBS and then lysed in a lysis buffer for 10 min on ice. The protein content of the cell lysates is assayed with a Micro BCA reagent. Cell lysates containing 50 μg of protein are incubated with a caspase-3 fluorogenic substrate (DEVD-AFC) or a caspase-9 fluorogenic substrate (LEHD-AFC) for 1 h at 37°C. Caspase activity is measured by fluorometric detection [3].
Cell Research Cells are seeded in 96-well plates at a density of 1×10^5 cells per well. The cells are maintained for 24 h at 37°C and then Warangalone (30 μM) is added to the culture medium. MTS solution is added to the 96-well plates at the indicated time points, and the cells are incubated for 1 h at 37°C. The absorbance is measured at a wavelength of 490 nm with a microplate counter [3].
Source
Synonyms Scandenolone
Molecular Weight 404.46
Formula C25H24O5
CAS No. 4449-55-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: Soluble

TargetMolReferences and Literature

1. Tati Herlina, et al. ANTI-MALARIAL COMPOUND FROM THE STEM BARK OF Erythrina variegate. Indo. J. Chem., 2009, 9 (2), 308-311. 2. Wang BH, et al. Specific inhibition of cyclic AMP-dependent protein kinase by warangalone and robustic acid. Phytochemistry. 1997 Mar;44(5):787-96. 3. Induction of apoptosis by isoflavonoids from the leaves of Millettia taiwaniana in human leukemia HL-60 cells. Planta Med. 2006 Apr;72(5):424-9.

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Keywords

Warangalone 4449-55-2 Tyrosine Kinase/Adaptors PKA Scandenolone inhibitor inhibit

 

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