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SecinH3

Catalog No. T6664 CAS 853625-60-2

SecinH3 is selective cytohesin inhibitor.

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SecinH3, CAS 853625-60-2

Pack Size	Availability	Price/USD
1 mg	In stock	$ 33.00
5 mg	In stock	$ 72.00
10 mg	In stock	$ 126.00
25 mg	In stock	$ 238.00
50 mg	In stock	$ 425.00
100 mg	In stock	$ 628.00
1 mL * 10 mM (in DMSO)	In stock	$ 76.00

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Purity: 98.14%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	SecinH3 is selective cytohesin inhibitor.
Targets&IC50	Cyh3 (human):5.6 μM, Cyh1 (human):5.4 μM, Cyh3 (mouse):5.4 μM, Cyh2 (human):2.4 μM, Drosophila steppke:5.6 μM
In vitro	In HepG2 cells, SecinH3 inhibits insulin signaling and associated gene expression. [1] SecinH3 also markedly inhibits migration of preadipocyte 3T3-L1 cells. [2] In the A549 cells, SecinH3 results in reduced proliferation by indirectly attenuating EGFR activation, and causes a synergistic antiproliferative effect when used in combination with gefitinib. [3]
In vivo	In mice, SecinH3 increases the expression of gluconeogenic genes, reduces the expression of glycolytic, fatty acid and ketone body metabolism genes in the liver, reduces liver glycogen stores, and increases plasma insulin. [1] In mice bearing H460 xenografts, SecinH3 significantly retards tumor growth through its antiproliferative and pro-apoptotic effect. [3]
Kinase Assay	Aptamer displacement screen: A library of small molecules screened in duplicate from Comgenex (10000 molecules) in 386 well plates by fluorescence polarization at an excitation wavelength of 485 nm using read-out at 520 nm in a reaction volume of 50 μL at 37° C. The screening buffer is PBS, pH 7.5, 3 mM MgCl2, 100 nM fluorecein-labelled M69 aptamer, 1 μM cytohesin-1 Sec7 at 100 μM compound concentrations.
Cell Research	3×103 cells per 96 well are seeded into a clear, flat bottom 96 well plate (TPP). After 24 h the cells are treated with the indicated concentrations of the inhibitors or solvent (final DMSO concentration 0.4%) in RPMI containing 50 ng/mL EGF or IGF-1, respectively. Medium is changed daily for 3 days and cell proliferation is analyzed with a MTT assas as described in the manufacturer's protocol using a Varioscan microplate reader. All assays are performed at least in triplicates. (Only for Reference)

Molecular Weight	460.51
Formula	C24H20N4O4S
CAS No.	853625-60-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 4.6 mg/mL (10 mM)), Heating is recommended.

DMSO: 46.1 mg/mL (100 mM)

References and Literature

1. Hafner M, et al. Nature. 2006, 444(7121), 941-944. 2. Torii T, et al. J Biol Chem. 2010, 285(31), 24270-24281. 3. Bill A, et al. PLoS One. 2012, 7(7), e41179.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compound Library NO PAINS Compound Library Anti-Aging Compound Library Oxidation-Reduction Compound Library Apoptosis Compound Library Bioactive Compounds Library Max

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SecinH3 853625-60-2 Apoptosis SecinH 3 inhibit SecinH-3 Inhibitor inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.