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Cell Cycle/Checkpoint PLK SBE 13 hydrochloride

SBE 13 hydrochloride

Catalog No. T2271   CAS 1052532-15-6
Synonyms: SBE 13 HCl

SBE13 hydrochloride is an effective and specific PLK1 inhibitor (IC50: 0.2 nM); no inhibition om Aurora A kinase, Plk2/3.

SBE 13 hydrochloride, CAS 1052532-15-6
Pack Size Availability Price/USD Quantity
5 mg In stock 60.00
10 mg In stock 95.00
25 mg In stock 190.00
50 mg In stock 350.00
100 mg In stock 595.00
1 mL * 10 mM (in DMSO) In stock 60.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description SBE13 hydrochloride is an effective and specific PLK1 inhibitor (IC50: 0.2 nM); no inhibition om Aurora A kinase, Plk2/3.
Targets&IC50 PLK1 : ic50 200pM,   PLK2 : ic50 >66μM,   PLK3 : ic50 875nM,  
Kinase Assay Kinase assays: To assay Plk1 and Aurora A kinase activity, cells are lysed after 13 hrs release in the presence of SBE13 after double thymidine block, and kinases are immunoprecipitated from lysates using antibodies as described. In brief, for each immunoprecipitation 800 µg of total protein were incubated with 1.5 µg Plk1 antibody cocktail, 3 µg Plk2 antibody, 3 µg Plk3 antibody, or 5 µg Aurora A antibody, respectively, for 2 hrs at 4°C on a rotator. Immunoprecipitated protein is collected using Protein G Agarose beads. The Plk1, Plk2 and Plk3 immunoprecipitates are incubated with 1 µg casein and with 1 µCi of [γ32-P]ATP for 30 min at 37°C in kinase buffer. The Aurora A immunoprecipitates are incubated with 0.5 µl Histone and with 1 µCi of [γ32-P]ATP for 60 min at room temperature in kinase buffer. Products from the kinase assays are fractionated on 10% Bis-Tris-polyacrylamide gels, and the phosphorylated substrate is visualized by autoradiography after an exposure of 12 to 36 hrs. An equal amount of immunoprecipitates is subjected to western blot analysis to confirm equal loading of Plk1, Plk2, Plk3 or Aurora A protein in kinase reactions. Immunoprecipitated Plk1 after 13 hrs release in the presence of SBE13 is assayed after de-phosphorylation using λ protein phosphatase and compared to kinase activity of endogenous immunoprecipitated Plk1. Activity of Plk1 kinase with and wiiiuithout de-phosphorylation is compared and the ratio between de-phosphorylated and “normal” endogenous immunoprecipitated Plk1 kinase activity is calculated.
Cell Research
Cells are treated with SBE13 one day after subculturing. Control cells are incubated with normal culture medium. Concentrations of SBE13 ranged from 1 nM–100 µM. The growth rate of 1 x 105 cells per 6-well is determined by counting cells at 24, 48 and 72 hours after treatment. Cell culture studies are performed in triplicate for each time point.(Only for Reference)
Cell lines: HeLa, A431, HCT-15, HT-29, MCF-7, U2OS, LN-229, SKW 6.4, PC-3, Det-562, SK-BR-3, SK-OV-3 and A549nb cells
Synonyms SBE 13 HCl
Purity 98.00%
Molecular Weight 479.4
Formula C24H28Cl2N2O4
CAS No. 1052532-15-6

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 47.9 mg/mL (100 mM)

water: 4.8 mg/mL (10 mM)), with gentle warming

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Keppner S, et al. Cell Cycle. 2010, 9(4), 761-773. 2. Keppner S, et al. Cell Cycle. 2011, 10(4), 708-720. 3. Lange L, et al. Oncotarget. 2014, 5(8), 2263-2275.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Apoptosis Compound Library Autophagy Compound Library DNA Damage & Repair Compound Library Kinase Inhibitor Library Bioactive Lipid Compound Library Cell cycle related Compound Library

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