Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 47.00 | |
10 mg | In stock | $ 70.00 | |
25 mg | In stock | $ 113.00 | |
50 mg | In stock | $ 153.00 | |
100 mg | In stock | $ 279.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 77.00 |
Description | PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM). |
Targets&IC50 | p70 S6K1:160 nM |
Kinase Assay | Affinity determination: Purified activated FAK kinase domain (amino acids 410–689) is reacted with 50 μM ATP, and 10 μg/well of a random peptide polymer of Glu and Tyr (molar ratio of 4:1), poly(Glu/Tyr) in kinase buffer (50 mM HEPES, pH 7.5, 125 mM NaCl, 48 mM MgCl2) for 15 min. Phosphorylation of poly(Glu/Tyr) is challenged with serially diluted compounds at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is run in triplicate. Phosphorylation of poly(Glu/Tyr) is detected with a general anti-phospho-tyrosine (PY20) antibody, followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody. The standard horseradish peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine is added, and Optical Density readings at 450 nm are obtained following the addition of stop solution (2 M H2SO4). The IC50 values are determined using the Hill slope model. |
Synonyms | PF4708671 |
Molecular Weight | 390.41 |
Formula | C19H21F3N6 |
CAS No. | 1255517-76-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 19.5 mg/mL (50 mM)
Ethanol: 19.5 mg/mL (50 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
PF-4708671 1255517-76-0 Autophagy MAPK PI3K/Akt/mTOR signaling S6 Kinase inhibit S6K Inhibitor PF4708671 Ribosomal S6 Kinase (RSK) PF 4708671 inhibitor