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Oxymatrine

Catalog No. T2754 CAS 16837-52-8

Synonyms: Oxysophoridine, Matrine N-oxide

Oxymatrine (Oxysophoridine) is an alkaloid isolated from Sophora flavescens, used as the antibiotic. It is a traditional Chinese medicine used in the treatment against hepatitis B virus.It can also inhibit iNOS expression and TGF - β / Smad pathway.

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Oxymatrine, CAS 16837-52-8

Pack Size	Availability	Price/USD
50 mg	In stock	$ 41.00
100 mg	In stock	$ 59.00
200 mg	In stock	$ 89.00
500 mg	In stock	$ 128.00
1 g	In stock	$ 178.00
1 mL * 10 mM (in DMSO)	In stock	$ 65.00

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Purity: 99.84%

Biological Description

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Storage & Solubility Information

Description	Oxymatrine (Oxysophoridine) is an alkaloid isolated from Sophora flavescens, used as the antibiotic. It is a traditional Chinese medicine used in the treatment against hepatitis B virus.It can also inhibit iNOS expression and TGF - β / Smad pathway.
In vivo	LD50 in mouse: 521 mg/kg, i.p.
Kinase Assay	P450 Inhibition Studies: Pooled human liver microsomes (HLM) from at least 15 donors are used for all inhibition assays. For IC50 determinations, the substrate probes are used at their approximate in vitro Km values. All incubations are performed with 100 mM potassium phosphate buffer (pH 7.4) and 1 mM NADPH. For CYP1A2 inhibition study, incubations are performed in a total volume of 0.5 ml, in duplicates with 0.1 mg/ml HLM, 30 μM phenacetin, 1 mM NADPH, and in the presence of avasimibe (0, 0.3, 0.75, 1.5, 3, 7.5, 15, 30, and 40 μM in 50 mM) in a potassium phosphate buffer at pH 7.4. After preincubation at 37 °C for 7 min, NADPH is added to initiate the enzyme reaction. The reaction mixture is quenched with 500 μl of ice-cold 100 ng/ml paracetamol-D4/CH3CN after 25 min. The standards (4-acetamidophenol, singlet) and quality controls (triplicates for low, medium, and high) are prepared at room temperature. After mixing, 0.2 ml of the samples is transferred to another plate and submitted for LC/MS/MS analysis after centrifugation at 3000 rpm for 10 min. A Supelco Discovery Amide C16, 100 × 2.1 mm (5-μm particle size) column (Supelco, Bellefonte, PA) is used. The mobile phase is isocratic, 40:60 [acetonitrile/formic acid, 0.1% (v/v)] at 0.2 ml/min.
Cell Research	DU145, PC-3 and PNT1B cell lines (3×104 cells/well) are seeded into 96-well plates and incubated overnight at 37°C in 5% CO2. Subsequently, the cells are incubated with different concentrations of oxymatrine (0, 2, 4, 6 and 8 mg/mL). MTT (10 mL; 5 mg/mL) is added and the mixture is incubated in darkness at 37°C for 2 h. Absorbance is measured at a wavelength of 490 nm using a microplate reader[2].
Source	Natural Products > Fabaceae > Sophora

Synonyms	Oxysophoridine, Matrine N-oxide
Molecular Weight	264.36
Formula	C15H24N2O2
CAS No.	16837-52-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 100 mg/mL (378.27 mM), Sonification is recommended.

H2O: 33.33 mg/ml(126.09 mM), Sonification is recommended.

References and Literature

1. Xiang X, et al. Chin Med J (Engl), 2002, 115(4), 593-596. 2. Chen XS, et al. World J Gastroenterol, 2001, 7(1), 49-52.

Citations

1. Mlicka A, Zielińska W, Mikołajczyk K, et al.Synergistic effect of oxymatrine and 5-fluorouracil on the migratory potential in A549 non-small cell lung cancer cells.Medical Research Journal.2022, 7(4): 293-300.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Approved Drug Library Anti-Cancer Drug Library TGF-beta/Smad Compound Library Drug Repurposing Compound Library Inhibitor Library Anti-Cancer Active Compound Library Reprogramming Compound Library Cancer Cell Differentiation Compound Library ReFRAME Related Library Anti-Liver Cancer Compound Library

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Keywords

Oxymatrine 16837-52-8 Autophagy Microbiology/Virology Stem Cells Influenza Virus TGF-beta/Smad Oxysophoridine Inhibitor inhibit Transforming growth factor beta Matrine N-oxide inhibitor

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