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Oxymatrine

Oxymatrine
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Purity:99.84%
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Oxymatrine

Catalog No. T2754Cas No. 16837-52-8
Oxymatrine (Oxysophoridine) is an alkaloid isolated from Sophora flavescens, used as the antibiotic. It is a traditional Chinese medicine used in the treatment against hepatitis B virus.It can also inhibit iNOS expression and TGF - β / Smad pathway.
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Pack SizePriceAvailabilityQuantity
50 mg$41In Stock
100 mg$59In Stock
200 mg$89In Stock
500 mg$128In Stock
1 g$178In Stock
1 mL x 10 mM (in DMSO)$65In Stock
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Product Introduction

Bioactivity
Description
Oxymatrine (Oxysophoridine) is an alkaloid isolated from Sophora flavescens, used as the antibiotic. It is a traditional Chinese medicine used in the treatment against hepatitis B virus.It can also inhibit iNOS expression and TGF - β / Smad pathway.
In vivo
LD50 in mouse: 521 mg/kg, i.p.
Kinase Assay
P450 Inhibition Studies: Pooled human liver microsomes (HLM) from at least 15 donors are used for all inhibition assays. For IC50 determinations, the substrate probes are used at their approximate in vitro Km values. All incubations are performed with 100 mM potassium phosphate buffer (pH 7.4) and 1 mM NADPH. For CYP1A2 inhibition study, incubations are performed in a total volume of 0.5 ml, in duplicates with 0.1 mg/ml HLM, 30 μM phenacetin, 1 mM NADPH, and in the presence of avasimibe (0, 0.3, 0.75, 1.5, 3, 7.5, 15, 30, and 40 μM in 50 mM) in a potassium phosphate buffer at pH 7.4. After preincubation at 37 °C for 7 min, NADPH is added to initiate the enzyme reaction. The reaction mixture is quenched with 500 μl of ice-cold 100 ng/ml paracetamol-D4/CH3CN after 25 min. The standards (4-acetamidophenol, singlet) and quality controls (triplicates for low, medium, and high) are prepared at room temperature. After mixing, 0.2 ml of the samples is transferred to another plate and submitted for LC/MS/MS analysis after centrifugation at 3000 rpm for 10 min. A Supelco Discovery Amide C16, 100 × 2.1 mm (5-μm particle size) column (Supelco, Bellefonte, PA) is used. The mobile phase is isocratic, 40:60 [acetonitrile/formic acid, 0.1% (v/v)] at 0.2 ml/min.
Cell Research
DU145, PC-3 and PNT1B cell lines (3×104 cells/well) are seeded into 96-well plates and incubated overnight at 37°C in 5% CO2. Subsequently, the cells are incubated with different concentrations of oxymatrine (0, 2, 4, 6 and 8 mg/mL). MTT (10 mL; 5 mg/mL) is added and the mixture is incubated in darkness at 37°C for 2 h. Absorbance is measured at a wavelength of 490 nm using a microplate reader[2].
AliasOxysophoridine, Matrine N-oxide
Chemical Properties
Molecular Weight264.36
FormulaC15H24N2O2
Cas No.16837-52-8
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 100 mg/mL (378.27 mM), Sonication is recommended.
H2O: 33.33 mg/mL (126.09 mM), Sonication is recommended.
Solution Preparation Table
DMSO/H2O
1mg5mg10mg50mg
1 mM3.7827 mL18.9136 mL37.8272 mL189.1360 mL
5 mM0.7565 mL3.7827 mL7.5654 mL37.8272 mL
10 mM0.3783 mL1.8914 mL3.7827 mL18.9136 mL
20 mM0.1891 mL0.9457 mL1.8914 mL9.4568 mL
50 mM0.0757 mL0.3783 mL0.7565 mL3.7827 mL
100 mM0.0378 mL0.1891 mL0.3783 mL1.8914 mL

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