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Lithocholic acid

Catalog No. T2202 CAS 434-13-9

Synonyms: 3α-Hydroxy-5β-cholanic acid

Lithocholic acid is a bile acid formed from chenodeoxycholate by bacterial action, usually conjugated with glycine or taurine. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as cholagogue and choleretic.

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Lithocholic acid, CAS 434-13-9

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Kinase Assay

Competitive ligand binding assay.: Ligand binding is performed using lysates from COS-7 cells transfected with expression plasmids for VDR or RXRα. Binding is performed overnight at 4°C in lysate buffer with 0.71 nM (18 Ci/mmol) [3H]1,25(OH)2D3 and bile acid competitor. Unbound [3H]1,25(OH)2D3 is removed by adsorption to dextran-coated charcoal and the supernatant removed for scintillation counting. Ki values are calculated from a computer fit of competition curves from triplicate assays.

Synonyms	3α-Hydroxy-5β-cholanic acid
Molecular Weight	376.58
Formula	C24H40O3
CAS No.	434-13-9

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: 44 mg/mL (116.8 mM)

DMSO: 70 mg/mL (185.9 mM)

H2O: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Staudinger JL, et al. PNAS, 2001, 98(6), 3369-3374. 2. Parks DJ, et al. Science, 1999, 284(5418), 1365-1368. 3. Makishima M, et al. Science, 2002, 296(5571), 1313-1316. 4. Ogawa A, et al. Jpn J Cancer Res, 1998, 89(11), 1154-1159. 5. Kozoni V, et al. Carcinogenesis, 2000, 21(5), 999-1005. 6. Yang R, et al. Metabolomic analysis of cholestatic liver damage in mice. Food Chem Toxicol. 2018 Jul 14;120:253-260.

Citations

1. Yan Y, Niu Z, Sun C, et al. Hepatic thyroid hormone signalling modulates glucose homeostasis through the regulation of GLP-1 production via bile acid-mediated FXR antagonism. Nature Communications. 2022, 13(1): 1-16.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Lithocholic acid 434-13-9 Apoptosis Autophagy Metabolism Others Endogenous Metabolite FXR inhibit 3α-Hydroxy-5β-cholanic acid Inhibitor inhibitor

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