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LM22B-10

Catalog No. T4496   CAS 342777-54-2
Synonyms: LM22B10

LM22B-10 is an activator of TrkB/TrkC neurotrophin receptor and can induce TrkB, TrkC, <a href="/target/ERK" style="display: inline; color: #c13a36">ERK</a> and <a href="/target/Akt" style="display: inline; color: #c13a36">Akt</a> activation in vitro and in vivo.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
LM22B-10, CAS 342777-54-2
Pack Size Availability Price/USD Quantity
1 mg In stock $ 54.00
2 mg In stock $ 84.00
5 mg In stock $ 128.00
10 mg In stock $ 216.00
25 mg In stock $ 515.00
50 mg In stock $ 882.00
100 mg In stock $ 1,552.00
1 mL * 10 mM (in DMSO) In stock $ 135.00
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Purity: 99.13%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description LM22B-10 is an activator of TrkB/TrkC neurotrophin receptor and can induce TrkB, TrkC, ERK and AKT activation in vitro and in vivo.
In vitro LM22B-10 shows maximum neurotrophic survival activity levels that are higher than those maximally achieved with BDNF (53 ± 7.2% above BDNF at 0.7 nM) and NT-3 (91 ± 8.6% above NT-3 at 0.7 nM) with an EC50 value of 200-300 nM. LM22B-10 (1000 nM) induces neurites of significantly larger average lengths, up to -40 μM. LM22B-10 (250-2000 nM) binds to TrkB-Fc and TrkC-Fc in a dose-dependent manner. LM22B-10 inhibits binding of BDNF to TrkB-expressing cells and NT-3 to TrkC-expressing cells. LM22B-10 promotes cell survival and functions preferentially through TrkB and TrkC. LM22B-10, but not BDNF or NT-3, promotes neurite outgrowth in an inhibitory environment. LM22B-10 induces patterns of Trk and downstream signaling activation that are distinct from those of BDNF and NT-3. LM22B-10 also induces TrkB, TrkC, AKT and ERK activation in hippocampal neurons in culture[1].
In vivo LM22B-10 (0.5 mg/kg) activates TrkB, TrkC, AKT and ERK in C57BL/6J mice. LM22B-10 (50 mg/kg, i.p.) shows increased phosphorylation at TrkBY817 and TrkCY820. LM22B-10 activates synaptic TrkB and TrkC and increases pre- and post-synaptic proteins and spine density in aged mice [1].
Cell Research Mouse NIH-3T3 cells, mouse NIH-3T3 cells expressing TrkA (NIH-3T3-TrkA) or p75NTR (NIH-3T3-p75NTR), and NIH-3T3 cells expressing TrkB (NIH-3T3-TrkB) or TrkC (NIH-3T3-TrkC) are propagated in DMEM supplemented with 10% FBS and 200-400 μg/mL Geneticin (for Trk-expressing cells) or 400 μg/mL hygromycin (for p75NTR-expressing cells). Cells are seeded into 24-well plates (30,000 cells/well) and cultured in medium consisting of 50% PBS and 50% DMEM without supplements. Following exposure to growth factors (0.7 nM) or 1000 nM LM22B-10 for 72-96 h, cells are suspended in 50 μL lysis buffer, transferred to opaque 96-well culture plates and survival is measured using the ViaLight Assay.
Synonyms LM22B10
Molecular Weight 485.02
Formula C27H33ClN2O4
CAS No. 342777-54-2

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: 4.5 mg/mL

DMSO: 45 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Yang T, et al. A small molecule TrkB/TrkC neurotrophin receptor co-activator with distinctive effects on neuronal survival and process outgrowth. Neuropharmacology. 2016 Nov;110(Pt A):343-61.

Related compound libraries

This product is contained In the following compound libraries:
Anti-COVID-19 Compound Library Anti-Pancreatic Cancer Compound Library PI3K-AKT-mTOR Compound Library Angiogenesis related Compound Library Neural Regeneration Compound Library Anti-Obesity Compound Library Anti-Liver Cancer Compound Library Anti-Lung Cancer Compound Library MAPK Inhibitor Library Neuronal Differentiation Compound Library

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Keywords

LM22B-10 342777-54-2 Cytoskeletal Signaling MAPK PI3K/Akt/mTOR signaling Tyrosine Kinase/Adaptors Akt Trk receptor ERK inhibit Trk Receptor LM-22B-10 LM22B10 LM22B 10 Tropomyosin related kinase receptor PKB Inhibitor Protein kinase B Extracellular signal regulated kinases inhibitor