Anti-AKT1 Polyclonal Antibody is a Mouse antibody targeting AKT1. Anti-AKT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PKB Polyclonal Antibody, Unconjugated (TMAB-00087) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (human memmery cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKT1) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MAKT1) polyclonal Antibody, Unconjugated (TMAB-00087) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
4. Sample:
NIH/3T3 (Mouse) Cell Lysate at 30 μg
MCF-7 (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-AKT1 (TMAB-00087) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa
5. Tissue/cell: MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (TMAB-00087) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
6. Tissue/cell: MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (TMAB-00087) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
7. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MAKT1) polyclonal Antibody, Unconjugated (TMAB-00087) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG-CY3 antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
8. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (TMAB-00087) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKT1) Polyclonal Antibody, Unconjugated (TMAB-00087) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AKT1) Monoclonal Antibody, Unconjugated (TMAB-00087) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human AKT-1

Human

Metabolism,AKT,Nuclear,Apoptosis,PKB / AKT