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Anti-ERK1/2 Antibody (2U314)
😃Good
Catalog No. TMAB-00635
Anti-ERK1/2 Antibody (2U314) is a Mouse antibody targeting ERK1/2. Anti-ERK1/2 Antibody (2U314) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ERK1/2 Antibody (2U314)
Anti-ERK1/2 Antibody (2U314)
😃Good
Catalog No. TMAB-00635
Anti-ERK1/2 Antibody (2U314) is a Mouse antibody targeting ERK1/2. Anti-ERK1/2 Antibody (2U314) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Mouse,Rat (predicted:Human) |
| Ig Type | IgG |
| Clone | 2U314 |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1/2) Monoclonal Antibody, Unconjugated (TMAB-00635) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (TMAB-00635) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (TMAB-00635) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-ERK1/2 (TMAB-00635) at 1/1000 dilution Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific Predicted band size: 43 kDa Observed band size: 44,42 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase. PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-244 and Ser-246 as well asautophosphorylation at Thr-188 promote nuclear localization. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human ERK1/2 |
| Antigen Species | Human |
| Gene ID | 5595 |
| Uniprot ID | |
| Biology Area | Tangles & Tau,MAPK Pathway,Cytoplasmic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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