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Anti-Phospho-AKT (Ser473) Antibody (9L992)
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Catalog No. TMAB-01378
Alias RAC-ALPHA, RAC, PRKBA, PKB-ALPHA, PKB, Phospho-AKT (S473), p-AKT (Ser473), p-AKT (S473), AKT (p-Ser473), AKT (p-S473), AKT
Anti-Phospho-AKT (Ser473) Antibody (9L992) is a Mouse antibody targeting Phospho-AKT (Ser473). Anti-Phospho-AKT (Ser473) Antibody (9L992) can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-AKT (Ser473) Antibody (9L992)
Anti-Phospho-AKT (Ser473) Antibody (9L992)
😃Good
Catalog No. TMAB-01378Alias RAC-ALPHA, RAC, PRKBA, PKB-ALPHA, PKB, Phospho-AKT (S473), p-AKT (Ser473), p-AKT (S473), AKT (p-Ser473), AKT (p-S473), AKT
Anti-Phospho-AKT (Ser473) Antibody (9L992) is a Mouse antibody targeting Phospho-AKT (Ser473). Anti-Phospho-AKT (Ser473) Antibody (9L992) can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-AKT (Ser473) Antibody (9L992) is a Mouse antibody targeting Phospho-AKT (Ser473). Anti-Phospho-AKT (Ser473) Antibody (9L992) can be used in IHC-P,IHC-Fr,IF. |
| Alias | RAC-ALPHA, RAC, PRKBA, PKB-ALPHA, PKB, Phospho-AKT (S473), p-AKT (Ser473), p-AKT (S473), AKT (p-Ser473), AKT (p-S473), AKT |
| Ig Type | IgG |
| Clone | 9L992 |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (TMAB-01378) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (TMAB-01378) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (TMAB-01378) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of TMAB-01378) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of TMAB-01378) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (Human skin carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT (Ser473)) Monoclonal Antibody, Unconjugated (ascites of TMAB-01378) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:500-1000; IF: 1:500-1000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human AKT around the phosphorylation site of Ser473 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Metabolism,AKT,Nuclear,Apoptosis,PKB / AKT |
| Molecular Weight | Theoretical: 56 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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