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MAPK Raf Kobe0065

Kobe0065

Catalog No. T1876   CAS 436133-68-5

Kobe0065 is a novel and effective small-molecule compound inhibiting Ras–Raf interaction by SBDD; shows potent activity to competitively inhibit the binding of H-Ras·GTP to c-Raf-1 RBD with a Ki value of 46 ± 13 μM.

Kobe0065, CAS 436133-68-5
Pack Size Availability Price/USD Quantity
5 mg In stock 50.00
10 mg In stock 64.00
25 mg In stock 114.00
50 mg In stock 160.00
100 mg In stock 260.00
200 mg In stock 430.00
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Purity 98.00%
Purity 99.76%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Kobe0065 is a novel and effective small-molecule compound inhibiting Ras–Raf interaction by SBDD; shows potent activity to competitively inhibit the binding of H-Ras·GTP to c-Raf-1 RBD with a Ki value of 46 ± 13 μM.
Targets&IC50 Ras-Raf
In vivo Kobe0065 has antitumor activity. The compound-treated tumors show a prominent increase of apoptotic cells without any significant body weight loss[1].
Cell Research
NIH 3T3 cells are transfected with pEF-BOS-HA-H-Ras(G12V) or an empty vector and treated with the 2 and 20 μM compound or the vehicle (DMSO) in the presence of 2% FBS for 1 h. Cell lysate is subjected to detection of c-Raf-1 coimmunoprecipitated with an anti-H-Ras antibody and total c-Raf-1 by Western blotting with an anti-c-Raf-1 antibody. Immunoprecipitated H-Ras(G12V) are detected by an anti-HA antibody.(Only for Reference)
Cell lines: NIH 3T3 cells
Molecular Weight 449.79
Formula C15H11ClF3N5O4S
CAS No. 436133-68-5

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

Ethanol: 4.5 mg/mL (10 mM)), with gentle warming

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Shima F, et al. Proc Natl Acad Sci U S A. 2013, 110(20):8182-7.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Apoptosis Compound Library Autophagy Compound Library MAPK Inhibitor Library Kinase Inhibitor Library GPCR Compound Library Fluorochemical Library Anti-cancer Metabolism Compound Library TGF-beta/Smad Compound Library Anti-obesity Compound Library HIF-1 Signaling Pathway Compound Library Anti-Pancreatic Cancer Compound Library Glutamine Metabolism Compound Library

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