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KC7F2

Catalog No. T3169 CAS 927822-86-4

KC7F2 is a potent HIF-1 pathway inhibitor with potential anti-cancer activity.

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KC7F2, CAS 927822-86-4

Pack Size	Availability	Price/USD
5 mg	In stock	$ 43.00
10 mg	In stock	$ 52.00
25 mg	In stock	$ 93.00
50 mg	In stock	$ 173.00
100 mg	In stock	$ 255.00
200 mg	In stock	$ 332.00
1 mL * 10 mM (in DMSO)	In stock	$ 54.00

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Purity: 99.11%

Purity: 98.6%

Purity: 98%

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Description	KC7F2 is a potent HIF-1 pathway inhibitor with potential anti-cancer activity.
Targets&IC50	HIF1α:20 μM
In vitro	KC7F2 inhibits HRE-driven transcription and decreases HIF-1α protein levels in LN229-HRE-AP cells. KC7F2 shows a dose-response cytotoxicity with IC50 of approximately 15 to 25 μM in cancer cells MCF7, LNZ308, A549, U251 mg, and LN229. In D54 mg glioma cells, KC7F2 inhibits colony formation, especially under hypoxia. [1] In hypoxic microglial cultures, KC7F2 downregulates the expression of TfR and DMT, and reduces the HIF-1α mediated iron accumulation. [2]
In vivo	KC7F2 significantly reduces the latent period in the pentylenetetrazole kindling rat model and increases the rate of spontaneous recurrent seizures during the chronic stage in the lithium-pilocarpine rat model. [3]
Kinase Assay	HIF transcriptional activity assay: Cells are incubated at 37 in a humidified atmosphere containing 5% CO2 and 21% O2 (normoxia) or 1% O2 (hypoxia) in a hypoxia workstation. The LN229-HRE-AP reporter cell line for HIF transcriptional activity is created by stably transfecting LN229 cells with the pACN188 plasmid, which contains an alkaline phosphatase gene driven by six HREs derived from the VEGF gene.
Cell Research	Cells are seeded onto 96-well plates (4 × 103/well) and cultured under normoxic (21% O2) and hypoxic (1% O2) conditions with different concentrations of KC7F2 for 72 h or treated for various times with 20 μM KC7F2. For proliferation analysis, cells are fixed with 50% trichloroacetic acid for 1 h at 4°C, followed by staining with 0.4% sulforhodamine B dissolved in 1% acetic acid for 30 min at room temperature. Plates are washed five times with 1% acetic acid to remove unbound dye. Bound dye is dissolved by adding 10 mM unbuffered Tris base. Cell proliferation is calculated by measuring OD values at 564 nm using a spectrophotometer.(Only for Reference)

Molecular Weight	570.38
Formula	C16H16Cl4N2O4S4
CAS No.	927822-86-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 57 mg/mL (100 mM)

References and Literature

1. Narita T, et al. Clin Cancer Res. 2009, 15(19), 6128-6136. 2. Rathnasamy G, et al. Neuropharmacology. 2014, 77, 428-440. 3. Li J, et al. Synapse. 2014, 68(9), 402-409. 4. Yang X L, Zeng M L, Shao L, et al. NFAT5 and HIF-1α Coordinate to Regulate NKCC1 Expression in Hippocampal Neurons after Hypoxia-Ischemia[J]. Frontiers in Cell and Developmental Biology. 2019, 7: 339.

Citations

1. Chen X C, Tang G X, Luo W H, et al. Monitoring and Modulating mtDNA G-Quadruplex Dynamics Reveal Its Close Relationship to Cell Glycolysis. Journal of the American Chemical Society. 2021 2. Yang X L, Zeng M L, Shao L, et al. NFAT5 and HIF-1α Coordinate to Regulate NKCC1 Expression in Hippocampal Neurons after Hypoxia-Ischemia. Frontiers in Cell and Developmental Biology. 2019, 7: 339 3. Liu R, Tian Y, Wang J, et al.Visible light–initiated radical 1,3-difunctionalization of β, γ-unsaturated ketones.Science Advances.2022, 8(49): eabq8596.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Inhibitor Library Reprogramming Compound Library Glycometabolism Compound Library Angiogenesis related Compound Library Bioactive Compounds Library Max Metabolism Compound Library Glycolysis Compound Library Bioactive Compound Library NO PAINS Compound Library

Related Products

Related compounds with same targets

HIF-1α-IN-6 AKB-6899 β,β-Dimethylacrylshikonin EHP-101 Glucosamine sulfate JNJ-42041935 HIF-1 alpha (556-574) (TFA) Deoxyshikonin

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Keywords

KC7F2 927822-86-4 Angiogenesis Chromatin/Epigenetic Metabolism HIF HIF/HIF Prolyl-Hydroxylase HIFs Inhibitor Hypoxia-inducible factors inhibit HIF-PH KC-7F2 inhibitor

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