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Acriflavine Hydrochloride (Synonyms: Acriflavine HCl)

Name: Acriflavine Hydrochloride
Brand: TargetMol
SKU: T19832
Price: 41 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T19832 Copy Product Info

Purity: 99.8%

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Acriflavine Hydrochloride (Acriflavine HCl) is a HIF-1α inhibitor. It acts by targeting HIF-1α-mediated pathways and decreasing the level of PGK1, VEGF, and HIF-1α in vitro and in vivo.

Acriflavine Hydrochloride

Copy Product Info

😃Good

Catalog No. T19832

Synonyms Acriflavine HCl

Acriflavine Hydrochloride (Acriflavine HCl) is a HIF-1α inhibitor. It acts by targeting HIF-1α-mediated pathways and decreasing the level of PGK1, VEGF, and HIF-1α in vitro and in vivo.

Cas No. 8063-24-9

Pack Size	Price	USA Stock	Global Stock	Quantity
500 mg	$41	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.8%

Appearance:Solid

Color:Red

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COA HNMR LCMS

Product Introduction

Acriflavine Hydrochloride AI Summary

Acriflavine Hydrochloride is a multifaceted bioactive molecule with a partition coefficient (logD7.4) of 1.6, indicating moderate lipophilicity. It has demonstrated significant trypanocidal activity against Trypanosoma brucei, with increasing efficacy under higher concentrations and light exposure. Notably, it impacts the cross resistance of CHO cells resistant to colchicine (CHRC5) with a log CR value of 0.84. The compound exhibits strong antimicrobial properties against a variety of bacterial strains, including Escherichia coli, Enterobacter cloacae, methicillin-resistant Staphylococcus aureus (MRSA), and Burkholderia pseudomallei. Its Minimum Inhibitory Concentrations (MIC) against these strains vary widely, reflecting differential efficacy based on specific genetic and resistance profiles. For example, MICs against multidrug-resistant E. coli with mutated GyrA gene exceed 128.0 µg/mL, while against other strains, MICs range from 2.0 to 4.0 µg/mL. The compound also affects the AcrAB-TolC efflux pump system, with an MIC of 400.0 µg/mL in Escherichia coli, and demonstrates efficacy against MRSA, with MIC50 at 12.5 µg/mL and MIC90 at 50.0 µg/mL. Furthermore, Acriflavine Hydrochloride shows potent antiviral activity, including a 74.97% inhibition of SARS-CoV-2 induced cytotoxicity in Caco-2 cells at 10 µM, and a 98.59% inhibition of SARS-CoV-2 3CL-Pro protease at 20 µM. It also inhibits HIF-1 dimerization in HEK293 cells with an IC50 of 1000 nM. In addition to its antimicrobial and antiviral activities, Acriflavine Hydrochloride impacts protein interactions and enzymatic activities, including Menin-MLL, BRCT-Phosphoprotein, Caspase-1, and Cytochrome P450 3A4. It also influences cellular responses such as ER Stress Response and DNA re-replication and exhibits antagonistic effects on proteins like MgcRacGAP. Overall, Acriflavine Hydrochloride is a versatile compound with significant potential in antimicrobial, antiviral, and cellular bioactivity modulation applications..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Acriflavine Hydrochloride (Acriflavine HCl) is a HIF-1α inhibitor. It acts by targeting HIF-1α-mediated pathways and decreasing the level of PGK1, VEGF, and HIF-1α in vitro and in vivo.
Synonyms	Acriflavine HCl

Chemical Properties

Molecular Weight	259.73
Formula	C₁₄H₁₄ClN₃
Cas No.	8063-24-9
Smiles	C[N+]1=C2C=C(N)C=CC2=CC3=C1C=C(N)C=C3.[Cl-]
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (115.5 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8502 mL	19.2508 mL	38.5015 mL	192.5076 mL
5 mM	0.7700 mL	3.8502 mL	7.7003 mL	38.5015 mL
10 mM	0.3850 mL	1.9251 mL	3.8502 mL	19.2508 mL
20 mM	0.1925 mL	0.9625 mL	1.9251 mL	9.6254 mL
50 mM	0.0770 mL	0.3850 mL	0.7700 mL	3.8502 mL
100 mM	0.0385 mL	0.1925 mL	0.3850 mL	1.9251 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc