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ID-8

Catalog No. T2431 CAS 147591-46-6

Synonyms: ID 8, ID8

ID-8, a DYRK inhibitor, sustains embryonic stem cell self-renewal in long-term culture.

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ID-8, CAS 147591-46-6

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Purity: 98%

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Description	ID-8, a DYRK inhibitor, sustains embryonic stem cell self-renewal in long-term culture.
Kinase Assay	In Vitro Enzymatic Assay for Histone Deacetylases: In vitro activities of the 11 recombinant human zinc-dependent HDAC enzymes are detected by ?uorigenic release of 7-amino-4-methylcoumarin from substrate upon deacetylase enzymatic activity. A series of dilutions of the unique HDAC6 compound, tubacin, and SAHA are prepared with 10% DMSO in HDAC assay buffer, and 5 μL of the dilution was added to a 50-μL reaction so that the ?nal concentration of DMSO is 1% in all of the reactions. The enzymatic reactions are conducted in duplicate at 37 °C for 30 min in a 50-μL mixture containing HDAC assay buffer, 5 μg BSA, an HDAC substrate, an HDAC enzyme, and a test compound. After enzymatic reactions, 50 μL of 2× HDAC developer is added to each well, and the plate is incubated at room temperature for an additional 15 min. Fluorescence intensity is measured at an excitation of 360 nm and an emission of 460 nm using a Synergy microplate reader. Negative (no enzyme, no inhibitor, a drug with no HDAC inhibition activity) and positive controls (known HDAC inhibitor SAHA) are included in the assays. IC50 is determined at the drug concentration that results in 50% reduction of HDAC activity compared with the control.
Cell Research	Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. (Only for Reference)

Synonyms	ID 8, ID8
Molecular Weight	298.298
Formula	C16H14N2O4
CAS No.	147591-46-6

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 29.8 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Miyabayashi T, et al. Biosci Biotechnol Biochem. 2008 , 72(5), 1242-1248. 2. Hasegawa K, et al. Stem Cells Transl Med. 2012, 1(1), 18-28.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Inhibitor Library Cell Cycle Compound Library Kinase Inhibitor Library Stem Cell Differentiation Compound Library Anti-Aging Compound Library Bioactive Compound Library Tyrosine Kinase Inhibitor Library

Related Products

Related compounds with same targets

LDN-192960 Protein kinase inhibitors 1 GNF2133 ML167 INDY EHT 1610 CLK-IN-T3 AZ-Dyrk1B-33

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

ID-8 147591-46-6 蛋白酪氨酸激酶细胞周期 DYRK cell embryonic dual-specificity ESC Inhibitor inhibit ID 8 Dual specificity tyrosine phosphorylation regulated kinase stem regulated phosphorylation kinase ID8 tyrosine Dual specificity tyrosine regulated kinase inhibitor

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