Powder: -20°C for 3 years | In solvent: -80°C for 1 year
HAMNO (NSC-111847) is a protein interaction inhibitor of replication protein A (RPA).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 47.00 | |
10 mg | In stock | $ 80.00 | |
25 mg | In stock | $ 163.00 | |
50 mg | In stock | $ 297.00 | |
100 mg | In stock | $ 537.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 52.00 |
Description | HAMNO (NSC-111847) is a protein interaction inhibitor of replication protein A (RPA). |
Targets&IC50 | RPA: |
In vitro | HAMNO inhibits colony formation in both HNSCC cell lines. The combination of HAMNO with etoposide markedly inhibits colony formation to a greater degree than HAMNO alone. In UMSCC38 cells, HAMNO dose-dependently increased the occurs of pan-nuclear γ-H2AX staining. UMSCC38 and UMSCC11B cells have prominent γ-H2AX staining, particularly after incubation with HAMNO (20 μM). In UMSCC38 and OKF4 cells, HAMNO increased γ-H2AX staining, with the greatest increase in signal occurring in S-phase. |
In vivo | In mice, HAMNO inhibits the progression of UMSCC11B tumors. After treatment with etoposide (20 μM, 2 h), Ser33 of RPA32 (an ATR substrate) is highly phosphorylated, which is reduced with the addition of 2 μM HAMNO and is nearly absent at higher concentrations, demonstrating HAMNO can inhibit RPA32 phosphorylation by ATR. |
Cell Research | Cell cycle assessment and γ-H2AX staining are monitored in UMSCC38 and OKF4 cells after 2 h incubation with HAMNO (2, 20, 50 μM) and fixed in 70% ethanol overnight. Cells are washed with PBS and incubated overnight in PBS containing 1% BSA, 10% goat serum, and PS139-H2AX antibodies washed and incubated in goat anti-mouse Alexa Fluor 647 antibody for 30 min at room temperature. Cells are incubated in 50 μg/mL propidium iodide and 100 μg/mL RNase A for 30 min, and 10,000 cells per sample are analyzed. |
Animal Research | UMSCC38 and UMSCC11B cells are implanted into 6-week-old female mice by a single subcutaneous injection of tumor cells (2 to 6×105 cells in 100 mL of sterile PBS). The growth rates of tumors are determined by daily monitoring of tumor volume with vernier calipers [tumor volume=1/2(length×width2)]. Once the tumor size reaches 50 mm3, etoposide (10 mg/kg mouse) and HAMNO (2 mg/kg) are administered intraperitoneally every day for 3 days. Tumor size is monitored daily and the volume of the tumor is compared among all experimental groups. |
Synonyms | NSC111847 |
Molecular Weight | 263.29 |
Formula | C17H13NO2 |
CAS No. | 138736-73-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 155 mg/mL
You can also refer to dose conversion for different animals. More
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HAMNO 138736-73-9 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis inhibit NSC111847 Inhibitor NSC 111847 NSC-111847 inhibitor