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Cetuximab

Catalog No. T9905 CAS 205923-56-4

Synonyms: Cetuximab (anti-EGFR), C225

Cetuximab (C225) is a monoclonal antibody that is an inhibitor of human epidermal growth factor receptor (EGFR) (Kd=0.201 nM). Cetuximab has antitumor activity, inhibiting tumor cell proliferation and inducing apoptosis.

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Cetuximab, CAS 205923-56-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 197.00
2 mg	In stock	$ 289.00
5 mg	In stock	$ 482.00
10 mg	In stock	$ 697.00
25 mg	In stock	$ 1,090.00
50 mg	In stock	$ 1,470.00
100 mg	In stock	$ 1,980.00

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AntibodyUnit: 5 mg/mL

Purity: 100.00%

AntibodyUnit: 5 mg/mL

Purity: 100.00%

AntibodyUnit: 5 mg/mL

Purity: 98.60%

AntibodyUnit: 5.2 mg/mL

Purity: 98.00%

AntibodyUnit: 5 mg/mL

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Description	Cetuximab (C225) is a monoclonal antibody that is an inhibitor of human epidermal growth factor receptor (EGFR) (Kd=0.201 nM). Cetuximab has antitumor activity, inhibiting tumor cell proliferation and inducing apoptosis.
Targets&IC50	EGFR:0.147 nM (Kd, Fixed A431 cells) , EGFR:0.201 nM (Kd)
In vitro	METHODS: Human squamous cell carcinoma (SCC) cells SCC-13Y, SCC-38, SCC-1, and SCC-11B were treated with Cetuximab (30 nM) for 8 days, and cell numbers were measured using a hemacytometer. RESULTS: Cetuximab inhibited cell proliferation of SCC cells in a time-dependent manner. Cetuximab inhibited the growth of SCC cell lines in a time-dependent manner, ranging from 20%-75% compared to untreated controls. [1] METHODS: EGFR mutant cells PC-9 and EGFR wild-type cells PC-14, A549 were treated with Cetuximab (10-100 µg/mL) for 24 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: EGFR phosphorylation was strongly expressed in PC-9 and continued to be strongly expressed during Cetuximab treatment.In PC-14 and A549 cells, although the increase in EGFR phosphorylation was reduced by the addition of Cetuximab, phosphorylation was not completely inhibited at the highest concentration. [2]
In vivo	METHODS: To assay antitumor activity in vivo, Cetuximab (1 mg/injection) was administered intraperitoneally to BALB/c (nu/nu) mice harboring HNSCC tumors UT-SCC-2 or UT-SCC-14 on the 10th, 13th, and 16th days after tumor cell injection. RESULTS: Cetuximab treatment reduced tumor growth in HNSCC xenografts and increased local oxygen partial pressure in tumors. [3] METHODS: To study in vivo antitumor activity, Cetuximab (0.25-1 mg/mouse) was administered intraperitoneally to nude mice bearing xenograft tumors every three days for a minimum of five injections. RESULTS: Treatment with Cetuximab alone effectively delayed the growth of GEO and L2987 tumors for at least 10 days. Borderline activity was observed in A549 and WiDr xenografts. However, Cetuximab did not show any significant anti-tumor activity in HT29, HCT116, LOVO, Colo205, LX-1, HCC70 and N87 models. [4]

Synonyms	Cetuximab (anti-EGFR), C225
Molecular Weight	152 kDa
Formula	C107H179N35O36S7
CAS No.	205923-56-4

Storage

store at low temperature

store at -20°C

References and Literature

1. Huang SM, et al. Epidermal growth factor receptor blockade with C225 modulates proliferation, apoptosis, and radiosensitivity in squamous cell carcinomas of the head and neck. Cancer Res. 1999 Apr 15;59(8):1935-40. 2. Kimura H, et al. Antibody-dependent cellular cytotoxicity of cetuximab against tumor cells with wild-type or mutant epidermal growth factor receptor. Cancer Sci. 2007 Aug;98(8):1275-80. 3. Gustafsson H, et al. EPR Oximetry of Cetuximab-Treated Head-and-Neck Tumours in a Mouse Model. Cell Biochem Biophys. 2017 Dec;75(3-4):299-309. 4. Wild R, et al. Cetuximab preclinical antitumor activity (monotherapy and combination based) is not predicted by relative total or activated epidermal growth factor receptor tumor expression levels. Mol Cancer Ther. 2006 Jan;5(1):104-13.

Citations

1. Komatsu M, Nakamura K, Takeda T, et al. Aurora kinase blockade drives de novo addiction of cervical squamous cell carcinoma to druggable EGFR signalling. Oncogene. 2022: 1-14.

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Keywords

Cetuximab 205923-56-4 Angiogenesis JAK/STAT signaling Tyrosine Kinase/Adaptors EGFR Inhibitor Epidermal growth factor receptor ErbB-1 inhibit Cetuximab (anti-EGFR) C225 C-225 C 225 anti-EGFR HER1 inhibitor

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