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Artesunate

Name: Artesunate
Brand: TargetMol
SKU: T0433
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: WR-256283) Copy Product Info

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Synonyms: WR-256283

Catalog No. T0433 Copy Product Info

Purity: 99.9%

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Artesunate (WR-256283) is a semisynthetic derivative of artemisinin and an inhibitor of STAT-3/EXP1. Water-soluble yet relatively unstable in neutral or acidic aqueous solutions, Artesunate is primarily employed for the treatment of malaria.

Cas No. 88495-63-0

Pack Size	Price	USA Stock	Global Stock
25 mg	$33	In Stock	In Stock
50 mg	$47	In Stock	In Stock
100 mg	$77	In Stock	In Stock
500 mg	$172	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$52	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.9%

Color:White

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	Artesunate (WR-256283) is a semisynthetic derivative of artemisinin and an inhibitor of STAT-3/EXP1. Water-soluble yet relatively unstable in neutral or acidic aqueous solutions, Artesunate is primarily employed for the treatment of malaria.
In vitro	Methods: SKM-1 cells were treated with 0–50 μg/mL Artesunate for 48 h. Methylation-specific PCR was used to detect CDH1 methylation status, while Western blot and laser confocal microscopy were employed to detect E-cadherin expression and localization. Results: Artesunate treatment induced CDH1 demethylation, restored E-cadherin expression, and promoted its aggregation to the cell membrane.[1] Methods: Human glioma SHG44 cells were treated with 0–50 mg/L Artesunate for 24–48 h. Cell proliferation was detected by MTT assay, apoptosis by flow cytometry, migration and invasion by Transwell assay, biomechanical properties by atomic force microscopy, and claudin-1 expression by RT-PCR and Western blot. Results: Treatment with 30 mg/L Artesunate for 48 h significantly inhibited cell proliferation, migration, and invasion, induced apoptosis rate to 23.7%, while increasing cell adhesion force and membrane roughness, decreasing elasticity, and upregulating claudin-1 expression.[2]
In vivo	Methods: HeLa cell xenograft nude mice were randomly divided into groups. The Artesunate group received daily intraperitoneal injection of 100 mg/kg (diluted in sterile PBS) for 7 consecutive days, while the irradiation group received 10 Gy irradiation on day 7. Results: Combined treatment reduced tumor volume by 72.34%, significantly higher than 41.22% with irradiation alone, confirming the in vivo radiosensitizing effect of ART.[3]
Synonyms	WR-256283
Cell Research	Growth inhibition assay: The SKM-1 cells (1×105/mL) are firstly seeded in 96-well plates. Artesunate is diluted in 0.1% dimethyl sulfoxide (DMSO) producing 0, 12.5, 25, 50µg/mL concentrations and added to the SKM-1 cells with 100 µl per well. A negative control is treated with 0.1% DMSO. At 0, 24, 48, and 72 hours, same amount of MTT solution is added into each well and cultured for extra 4 hours. MTT treated cells are fixed with 150 µL DMSO for 30 min at room temperature and then determined with Evolution 201 and 220 UV-Vis spectrophotometer system at 540 nm. (Only for Reference)

Chemical Properties

Molecular Weight	384.42
Formula	C₁₉H₂₈O₈
Cas No.	88495-63-0
Smiles	C[C@@H]1[C@]2([C@]34[C@](O[C@H]1OC(CCC(O)=O)=O)(O[C@](C)(OO3)CC[C@]4([C@H](C)CC2)[H])[H])[H]
Relative Density.	1.31 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 9 mg/mL (23.41 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 250 mg/mL (650.33 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (13.01 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6013 mL	13.0066 mL	26.0132 mL	130.0661 mL
5 mM	0.5203 mL	2.6013 mL	5.2026 mL	26.0132 mL
10 mM	0.2601 mL	1.3007 mL	2.6013 mL	13.0066 mL
20 mM	0.1301 mL	0.6503 mL	1.3007 mL	6.5033 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0520 mL	0.2601 mL	0.5203 mL	2.6013 mL
100 mM	0.0260 mL	0.1301 mL	0.2601 mL	1.3007 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.