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Aprotinin

Catalog No. T3359 CAS 9087-70-1

Synonyms: 抑酶肽, Antilysin, 抑肽酶, Bovine Pancreatic Trypsin Inhibitor, Traskolan

Aprotinin a broad-spectrum serine protease inhibitor, inhibiting the activity of a number of different esterases and proteases, including trypsin, chymotrypsin, kallikrein, plasmin, tissue plasminogen activator, and tissue and leukocytic proteinases.

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Aprotinin, CAS 9087-70-1

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Aprotinin a broad-spectrum serine protease inhibitor, inhibiting the activity of a number of different esterases and proteases, including trypsin, chymotrypsin, kallikrein, plasmin, tissue plasminogen activator, and tissue and leukocytic proteinases.
Targets&IC50	Trypsin:0.06 pM(Kd), kallikrein:0.8 nM(Kd), trypsinogen:2 μM(Kd), Chymotrypsin:9.5 nM(Kd)
In vitro	Aprotinin is an antifibrinolytic molecule that inhibits trypsin and related proteolytic enzymes. In cell biology, aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues. In the presence of aprotinin, the fibrinolytic activity is inhibited concentration dependently and the coagulation time is prolonged. Aprotinin is an effective inhibitor of the contact (intrinsic) coagulation pathway in vitro[2].
In vivo	Aprotinin inhibits clot lysis in vitro as well as rat-tail bleeding time in vivo and prolongs coagulation time in human plasma. In a rat arteriovenous shunt model, aprotinin reduces thrombus weight [2].
Kinase Assay	Substrates and kinases are diluted in 50?mM Tris/HCl (pH?7.5), 0.1% 2-mercaptoethanol, 0.1?mM EGTA and 10?mM magnesium acetate. Reactions are initiated with [γ-32P]ATP (2500 c.p.m./pmol) to a final concentration of 0.1?mM and terminated after 15?min at 30°C by the addition of SDS and EDTA (pH?7.0) to final concentrations of 1.0% (w/v) and 20?mM respectively. After heating for 5?min at 100°C and separation by SDS/PAGE, the phosphorylated proteins are detected by autoradiography.
Cell Research	Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days. (Only for Reference)

Synonyms	抑酶肽, Antilysin, 抑肽酶, Bovine Pancreatic Trypsin Inhibitor, Traskolan
Molecular Weight	6511.51
Formula	C284H432N84O79S7
CAS No.	9087-70-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 100 mg/mL (15.36 mM), Need ultrasonic

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Capasso C, et al. J Mol Recognit. 1997, 10(1):26-35. 2. Sperzel M, et al. J Thromb Haemost. 2007, 5(10):2113-2118. 3. James M. Wells, et al. Development. 1994, 120:3639-3647. 4. Jiang T Y, Feng X F, Fang Z, et al. PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer[J]. Cancer Letters. 2020 5. Jiang T Y, Pan Y F, Wan Z H, et al. PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma[J]. Science Translational Medicine. 2020, 12(562).

Citations

1. Jiang T Y, Pan Y F, Wan Z H, et al. PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. Science Translational Medicine. 2020, 12(562). 2. Jiang T Y, Feng X F, Fang Z, et al. PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer. Cancer Letters. 2021, 501: 187-199.

Related compound libraries

This product is contained In the following compound libraries：

HIF-1 Signaling Pathway Compound Library Peptide Compound Library Drug Repurposing Compound Library FDA-Approved Drug Library Anti-COVID-19 Compound Library Approved Drug Library FDA-Approved & Pharmacopeia Drug Library Bioactive Compound Library

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Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Aprotinin 9087-70-1 蛋白酶体泛素化其他微生物学 Influenza Virus Others Proteasome Serine Protease Ser/Thr Protease Inhibitor Antilysin Serine proteases Serine endopeptidases inhibit Threonine proteases Traskolan Bovine Pancreatic Trypsin Inhibitor inhibitor

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