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Proteases/Proteasome Proteasome Aprotinin

Aprotinin

Catalog No. T3359   CAS 9087-70-1
Synonyms: Bovine Pancreatic Trypsin Inhibitor, Antilysin, Traskolan

Aprotinin a broad-spectrum serine protease inhibitor, inhibiting the activity of a number of different esterases and proteases, including trypsin, chymotrypsin, kallikrein, plasmin, tissue plasminogen activator, and tissue and leukocytic proteinases.

Aprotinin
CAS 9087-70-1
Pack Size Availability Price/USD Quantity
5 mg In stock 50.00
10 mg In stock 60.00
25 mg In stock 130.00
50 mg In stock 230.00
100 mg In stock 375.00
200 mg In stock 570.00
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Purity 98.0%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Aprotinin a broad-spectrum serine protease inhibitor, inhibiting the activity of a number of different esterases and proteases, including trypsin, chymotrypsin, kallikrein, plasmin, tissue plasminogen activator, and tissue and leukocytic proteinases.
Targets&IC50 Chymotrypsin-like proteasome ,   kallikrein ,   Trypsin ,   trypsinogen
In vitro Aprotinin is an antifibrinolytic molecule that inhibits trypsin and related proteolytic enzymes. In cell biology, aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues. In the presence of aprotinin, the fibrinolytic activity is inhibited concentration dependently and the coagulation time is prolonged. Aprotinin is an effective inhibitor of the contact (intrinsic) coagulation pathway in vitro[2].
In vivo Aprotinin inhibits clot lysis in vitro as well as rat-tail bleeding time in vivo and prolongs coagulation time in human plasma. In a rat arteriovenous shunt model, aprotinin reduces thrombus weight [2].
Cell Research
Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days. (Only for Reference)
Cell lines: mouse G8-1 and C2C12 skeletal muscle myoblasts
Synonyms Bovine Pancreatic Trypsin Inhibitor , Antilysin , Traskolan
Purity 98.0%
Molecular Weight 6511.44
Formula C284H432N84O79S7
CAS No. 9087-70-1

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

H2O: 100 mg/mL (15.36 mM), Need ultrasonic

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
4. Jiang T Y, Feng X F, Fang Z, et al. PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer[J]. Cancer Letters. 2020 5. Jiang T Y, Pan Y F, Wan Z H, et al. PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma[J]. Science Translational Medicine. 2020, 12(562).

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library HIF-1 Signaling Pathway Compound Library Anti-COVID-19 Compound Library FDA Approved & Pharmacopeial Drug Library Peptide Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.