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6RK73

Name: 6RK73
Brand: TargetMol
SKU: T10188
Price: 147 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T10188Cas No. 1895050-66-4

6RK73 is a covalent, irreversible, and specific UCHL1 inhibitor (IC50: 0.23 µM) with almost no inhibition of UCHL3 (IC50: 236 µM).

6RK73

🥰Excellent

Purity: 99.91%

Catalog No. T10188Cas No. 1895050-66-4

6RK73 is a covalent, irreversible, and specific UCHL1 inhibitor (IC50: 0.23 µM) with almost no inhibition of UCHL3 (IC50: 236 µM).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$147	In Stock	In Stock
5 mg	$313	In Stock	In Stock
10 mg	$537	In Stock	In Stock
25 mg	$869	In Stock	In Stock
50 mg	$1,190	In Stock	In Stock
100 mg	$1,590	In Stock	In Stock
500 mg	$3,220	-	In Stock
1 mL x 10 mM (in DMSO)	$345	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Batch Information

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Purity:99.91%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	6RK73 is a covalent, irreversible, and specific UCHL1 inhibitor (IC50: 0.23 µM) with almost no inhibition of UCHL3 (IC50: 236 µM).
Targets&IC50	UCHL3:236 µM , UCHL1:0.23 µM
In vitro	6RK73 (5 µM; 1-3 hours) treatment displays strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels in MDA-MB-436 cells. 6RK73 (5 µM; 24-48 hours) results in migration significantly slower than the DMSO control group in MDA-MB-436 cells[1].
In vivo	6RK73 displays a potent inhibition of breast cancer extravasation in zebrafish[1].
Cell Research	Cell Line: MDA-MB-436 cells Concentration: 5 µM Incubation Time: 24, 48 hours

Chemical Properties

Molecular Weight	307.37
Formula	C₁₃H₁₇N₅O₂S
Cas No.	1895050-66-4
Smiles	O=C(Nc1ncc(s1)N1CCOCC1)[C@H]1CCN(C1)C#N
Relative Density.	1.41 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 112.5 mg/mL (366 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (13.01 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2534 mL	16.2670 mL	32.5341 mL	162.6704 mL
5 mM	0.6507 mL	3.2534 mL	6.5068 mL	32.5341 mL
10 mM	0.3253 mL	1.6267 mL	3.2534 mL	16.2670 mL
20 mM	0.1627 mL	0.8134 mL	1.6267 mL	8.1335 mL
50 mM	0.0651 mL	0.3253 mL	0.6507 mL	3.2534 mL
100 mM	0.0325 mL	0.1627 mL	0.3253 mL	1.6267 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc