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5-Bromouridine

Catalog No. T36894 CAS 957-75-5

5-Bromouridine is a brominated analog of the nucleoside uridine .1It can be incorporated into RNA and subsequently detected by antibodies against bromodeoxyuridine .25-Bromouridine decreases the viability of HL-60 and MOLT-4 cells (LC50s = 10 and 20 μM, respectively). It induces apoptosis and halts the cell cycle at the S phase in HL-60 cells. It is photoreactive, and UV irradiation has been used to cross-link RNA containing 5-bromouridine to proteins in the study of of RNA-protein interactions.1,35-Bromouridine can also be incorporated into RNA using 5-bromouridine 5'-triphosphate .

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5-Bromouridine, CAS 957-75-5

Pack Size	Availability	Price/USD	Quantity
500 mg	5 days	$ 41.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

Molecular Weight	323.1
Formula	C9H11BrN2O6
CAS No.	957-75-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

PBS (pH 7.2): 5 mg/mL

DMSO: 15 mg/mL

DMF: 10 mg/mL

References and Literature

1. Tanner, N.K., Hanna, M.M., and Abelson, J.Binding interactions between yeast tRNA ligase and a precursor transfer ribonucleic acid containing two photoreactive uridine analoguesBiochemistry27(24)8852-8861(1988) 2. Li, X., Patel, R., Melamed, M.R., et al.The cell cycle effects and induction of apoptosis by 5-bromouridine in cultures of human leukaemic MOLT-4 and HL-60 cell lines and mitogen-stimulated normal lymphocytesCell Prolif.27(6)307-319(1994) 3. Gott, J.M., Willis, M.C., Koch, T.H., et al.A specific, UV-induced RNA-protein cross-link using 5-bromouridine-substituted RNABiochemistry30(25)6290-6295(1991)

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

5-Bromouridine 957-75-5 5 Bromouridine 5Bromouridine inhibitor inhibit

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