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4-Aminohippuric Acid

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Catalog No. T1436Cas No. 61-78-9
Alias p-Aminohippuric acid

4-Aminohippuric Acid is the glycine amide of 4-aminobenzoic acid. Its sodium salt is used as a diagnostic aid to measure effective renal plasma flow (ERPF) and excretory capacity.

4-Aminohippuric Acid

4-Aminohippuric Acid

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Purity: 98.89%
Catalog No. T1436Alias p-Aminohippuric acidCas No. 61-78-9
4-Aminohippuric Acid is the glycine amide of 4-aminobenzoic acid. Its sodium salt is used as a diagnostic aid to measure effective renal plasma flow (ERPF) and excretory capacity.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 mg$35In StockIn Stock
100 mg$47In StockIn Stock
200 mg$68In StockIn Stock
1 mL x 10 mM (in DMSO)$50In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:98.89%
Appearance:Solid
Color:White
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COA HNMR LCMS HPLC

Product Introduction

Bioactivity
Description
4-Aminohippuric Acid is the glycine amide of 4-aminobenzoic acid. Its sodium salt is used as a diagnostic aid to measure effective renal plasma flow (ERPF) and excretory capacity.
In vitro
In normal individuals, the renal extraction ratio of 4-Aminohippuric acid is approximately 0.92. After extraction, the majority of 4-Aminohippuric acid is actively secreted by the proximal tubules and completely excreted by the kidneys. The clearance of 4-Aminohippuric acid is utilized to measure renal plasma flow and to assess the secretory mechanisms or transport limits of renal tubules. The kinetics of 4-Aminohippuric acid uptake by cortical tubular cells have been measured using the stopped-flow microperfusion technique in rat kidneys. In a rat model of renal failure, intravenous injection of 3.0 mg/kg Uranyl nitrate significantly reduced the CLPAH to 0.89 mL/min/kg, compared to 29.30 mL/min/kg in normal rats. Rats with chronic kidney failure exhibited altered pharmacokinetics, showing decreased renal excretion of 4-Aminohippuric acid. Compared to Sham rats, those with chronic kidney failure demonstrated a weakened load of excretion, filtration, and secretion of 4-Aminohippuric acid, with about a 40% reduction in the expression of OAT1 in the homogenate and membrane. Hence, measuring the renal clearance of 4-Aminohippuric acid typically reflects the kidney's capacity for organic anion transport.
In vivo
At a concentration of 10 mmol/L, 4-Aminohippuric acid was transported across membranes of HEK293 cells expressing MRP2 with a rate of 21.9 pmol/mg protein/min. The kinetic parameters for 4-Aminohippuric acid transport included a Km value of 880 mM and a Vmax value of 2.3 nmol/mg protein/min, indicating facilitated transport through organic anion transporters expressed in epithelial cells. Additionally, 4-Aminohippuric acid uptake by HEK293 cells, mediated by overexpressed and transfected NPT1, exhibited saturable kinetics in line with Michaelis-Menten dynamics, having a Km close to 2.66 mM and a Vmax of 940 pmol/mg protein/30s. This compound is also transported by transporters other than OAT, including NPT1 and MRP2.
Synonymsp-Aminohippuric acid
Chemical Properties
Molecular Weight194.19
FormulaC9H10N2O3
Cas No.61-78-9
SmilesNC1=CC=C(C=C1)C(=O)NCC(O)=O
Relative Density.1.356 g/cm3
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 50 mg/mL (257.48 mM), Sonication is recommended.
Ethanol: 2 mg/mL (10.3 mM), Sonication is recommended.
H2O: 3 mg/mL (15.45 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (10.3 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
Ethanol/H2O/DMSO
1mg5mg10mg50mg
1 mM5.1496 mL25.7480 mL51.4960 mL257.4798 mL
5 mM1.0299 mL5.1496 mL10.2992 mL51.4960 mL
10 mM0.5150 mL2.5748 mL5.1496 mL25.7480 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitEndogenous MetaboliteAminoAcidsandDerivativesAmino Acids and Derivatives4-Aminohippuric Acid4Aminohippuric Acid4 Aminohippuric Acid
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