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XMD17-109

Name: XMD17-109
Brand: TargetMol
SKU: T1842
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1842Cas No. 1435488-37-1

Alias XMD17 109, ERK5-IN-1

XMD17-109 (ERK5-IN-1) is a new selective ERK-5 inhibitor (EC50: 4.2 uM, HEK293 cells).

XMD17-109

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Purity: 99.7%

Catalog No. T1842Alias XMD17 109, ERK5-IN-1Cas No. 1435488-37-1

XMD17-109 (ERK5-IN-1) is a new selective ERK-5 inhibitor (EC50: 4.2 uM, HEK293 cells).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$35	In Stock	In Stock
2 mg	$48	In Stock	In Stock
5 mg	$79	In Stock	In Stock
10 mg	$147	In Stock	In Stock
25 mg	$233	In Stock	In Stock
50 mg	$377	In Stock	In Stock
100 mg	$567	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$112	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.7%

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	XMD17-109 (ERK5-IN-1) is a new selective ERK-5 inhibitor (EC50: 4.2 uM, HEK293 cells).
Targets&IC50	ERK5:162 nM(EC50), LRRK2 (G2019S):339 nM
In vivo	At the cellular level, ERK5-IN-1 inhibits endothelial growth factor-induced ERK5 autophosphorylation (EC50: 90 nM). It also effectively inhibits the biochemical properties of ERK5 (IC50: 162 nM).
Cell Research	HeLa cells are maintained in DMEM supplemented with 10% FBS, 2 mM?l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. Before use HeLa cells are serum starved for 16 h in DMEM supplemented with 2 mM?l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. HeLa cells are then incubated with ERK5-IN-1 at the indicated concentrations for 1 h prior to stimulation with 0.5mol/Lsorbitol for 30 min. Cells are lysed in Triton lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM EGTA, 1 mM EDTA, 1 mM sodium orthovanadate, 50 mM sodium fluoride, 1 mM sodium pyrophosphate, 0.27mol/Lsucrose, 1 μM microcystin-LR, 1% (v/v) Triton X-100, 0.1% (v/v) 2-mercaptoethanol) and 20 μg of protein loaded per well. Samples are run on 8% polyacrylamide gels using standard methods. Proteins are transferred onto nitrocellulose membranes and specific proteins detected by immunoblotting.
Synonyms	XMD17 109, ERK5-IN-1

Chemical Properties

Molecular Weight	638.8
Formula	C₃₆H₄₆N₈O₃
Cas No.	1435488-37-1
Smiles	CCOc1cc(ccc1Nc1ncc2N(C)C(=O)c3ccccc3N(C3CCCC3)c2n1)C(=O)N1CCC(CC1)N1CCN(C)CC1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 93 mg/mL (145.59 mM), Sonication is recommended.

Ethanol: 93 mg/mL (145.59 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (5.17 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.5654 mL	7.8272 mL	15.6544 mL	78.2718 mL
5 mM	0.3131 mL	1.5654 mL	3.1309 mL	15.6544 mL
10 mM	0.1565 mL	0.7827 mL	1.5654 mL	7.8272 mL
20 mM	0.0783 mL	0.3914 mL	0.7827 mL	3.9136 mL
50 mM	0.0313 mL	0.1565 mL	0.3131 mL	1.5654 mL
100 mM	0.0157 mL	0.0783 mL	0.1565 mL	0.7827 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc