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RO8994
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Catalog No. T3517Cas No. 1309684-94-3
RO8994 is a highly effective and specific inhibitor of spiroindolinone small-molecule MDM2, with IC50 of 20 nM (MTT proliferation assays) and 5 nM (HTRF binding assays).
RO8994
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Purity: 99.44%
Catalog No. T3517Cas No. 1309684-94-3
RO8994 is a highly effective and specific inhibitor of spiroindolinone small-molecule MDM2, with IC50 of 20 nM (MTT proliferation assays) and 5 nM (HTRF binding assays).
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $34 | In Stock | In Stock | |
| 2 mg | $48 | In Stock | In Stock | |
| 5 mg | $79 | In Stock | In Stock | |
| 10 mg | $129 | In Stock | In Stock | |
| 25 mg | $243 | In Stock | In Stock | |
| 50 mg | $383 | In Stock | In Stock | |
| 100 mg | $569 | In Stock | In Stock | |
| 1 mL x 10 mM (in DMSO) | $107 | In Stock | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Batch Information
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Purity:99.44%
Color:White
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Product Introduction
RO8994 AI Summary
RO8994 exhibits high oral bioavailability at 92.0% in C57 mice at a dosage of 25 mg/kg. It demonstrates rapid elimination, with a total plasma clearance of 5.8 mL.min-1.kg-1 and an apparent terminal half-life of 7.1 hours when administered intravenously at 0.64 mg/kg. The compound achieves a maximum concentration (Cmax) of 9453.64 nM in C57 mice at 25 mg/kg orally. In terms of antiproliferative activity, it has an IC50 of 5190.0 nM against human SW480 cells expressing p53 mutants and is significantly potent with an IC50 of 13.0 nM against human SJSA cells expressing wild-type p53. Additionally, RO8994 displays high binding affinity to Glutathione S-transferase-tagged MDM2 (IC50 = 5.0 nM), inhibiting its interaction with biotinylated p53 as measured by an HTRF assay. The compound shows some inhibitory activity against SARS-CoV-2 3CL-Pro protease with an inhibition percentage of 8.54%, and it inhibits SARS-CoV-2 induced cytotoxicity in VERO-6 cells with an approximate inhibition rate of 9.67% at 10 µM after 48 hours. Lastly, RO8994 exhibits cytotoxicity against human HepG2 cells with an IC50 of 5300.0 nM as assessed by the CellTiter-Glo luminescent cell viability assay..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | RO8994 is a highly effective and specific inhibitor of spiroindolinone small-molecule MDM2, with IC50 of 20 nM (MTT proliferation assays) and 5 nM (HTRF binding assays). |
| Targets&IC50 | MDM2:20 nM |
| Molecular Weight | 613.51 |
| Formula | C31H31Cl2FN4O4 |
| Cas No. | 1309684-94-3 |
| Smiles | COc1cc(ccc1NC(=O)[C@@H]1N[C@@H](CC(C)(C)C)[C@]2([C@H]1c1cccc(Cl)c1F)C(=O)Nc1cc(Cl)ccc21)C(N)=O |
| Relative Density. | 1.41 g/cm3 (Predicted) |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 50 mg/mL (81.5 mM), Sonication is recommended.  | ||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.26 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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