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NSC59984

Name: NSC59984
Brand: TargetMol
SKU: T6910
Price: 64 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T6910Cas No. 803647-40-7

NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation.

NSC59984

😃Good

Purity: 99.59%

Catalog No. T6910Cas No. 803647-40-7

NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$64	In Stock	In Stock
10 mg	$109	In Stock	In Stock
25 mg	$228	In Stock	In Stock
50 mg	$372	In Stock	In Stock
100 mg	$597	In Stock	In Stock
500 mg	$1,260	-	In Stock
1 mL x 10 mM (in DMSO)	$70	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.59%

Appearance:Solid

Color:White to Yellow

Resource Download

COA HNMR HPLC LCMS

Product Introduction

Bioactivity

Description	NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation.
In vitro	NSC59984 selectively reactivates the p53 pathway and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. It also promotes cell death in various cancer cell lines with different p53 mutations, showing minimal genotoxicity in normal cells. [1]
In vivo	In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner. [1]
Cell Research	Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.(Only for Reference)

Chemical Properties

Molecular Weight	265.27
Formula	C₁₂H₁₅N₃O₄
Cas No.	803647-40-7
Smiles	CN1CCN(CC1)C(=O)\C=C\c1ccc(o1)[N+]([O-])=O
Relative Density.	1.304 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 49 mg/mL (184.72 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 55 mg/mL (207.34 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7697 mL	18.8487 mL	37.6974 mL	188.4872 mL
5 mM	0.7539 mL	3.7697 mL	7.5395 mL	37.6974 mL
10 mM	0.3770 mL	1.8849 mL	3.7697 mL	18.8487 mL
20 mM	0.1885 mL	0.9424 mL	1.8849 mL	9.4244 mL
50 mM	0.0754 mL	0.3770 mL	0.7539 mL	3.7697 mL
100 mM	0.0377 mL	0.1885 mL	0.3770 mL	1.8849 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc