Shopping Cart

Your shopping cart is currently empty

NSC59984

Name: NSC59984
Brand: TargetMol
SKU: T6910
Price: 64 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T6910Cas No. 803647-40-7

NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation.

NSC59984

🥰Excellent

Purity: 99.59%

Catalog No. T6910Cas No. 803647-40-7

NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$64	In Stock	In Stock
10 mg	$109	In Stock	In Stock
25 mg	$228	In Stock	In Stock
50 mg	$372	In Stock	In Stock
100 mg	$597	In Stock	In Stock
500 mg	$1,260	-	In Stock
1 mL x 10 mM (in DMSO)	$70	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.59%

Resource Download

COA HNMR HPLC LCMS

Product Introduction

Bioactivity

Description	NSC59984, a p53 pathway activator, induces mutant p53 protein degradation and p73 activation.
In vitro	NSC59984 selectively reactivates the p53 pathway and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. It also promotes cell death in various cancer cell lines with different p53 mutations, showing minimal genotoxicity in normal cells. [1]
In vivo	In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner. [1]
Cell Research	Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.(Only for Reference)

Chemical Properties

Molecular Weight	265.27
Formula	C₁₂H₁₅N₃O₄
Cas No.	803647-40-7
Smiles	CN1CCN(CC1)C(=O)\C=C\c1ccc(o1)[N+]([O-])=O
Relative Density.	1.304 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 49 mg/mL (184.72 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 55 mg/mL (207.34 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7697 mL	18.8487 mL	37.6974 mL	188.4872 mL
5 mM	0.7539 mL	3.7697 mL	7.5395 mL	37.6974 mL
10 mM	0.3770 mL	1.8849 mL	3.7697 mL	18.8487 mL
20 mM	0.1885 mL	0.9424 mL	1.8849 mL	9.4244 mL
50 mM	0.0754 mL	0.3770 mL	0.7539 mL	3.7697 mL
100 mM	0.0377 mL	0.1885 mL	0.3770 mL	1.8849 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc