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KY1220

Name: KY1220
Brand: TargetMol
SKU: T15677
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15677Cas No. 292168-79-7

KY1220 destabilizes both β-catenin and Ras by targeting the Wnt/β-catenin pathway. It has an IC50 of 2.1 μM in HEK293 reporter cells.

KY1220

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Purity: 99.9%

Catalog No. T15677Cas No. 292168-79-7

KY1220 destabilizes both β-catenin and Ras by targeting the Wnt/β-catenin pathway. It has an IC50 of 2.1 μM in HEK293 reporter cells.

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$31	In Stock	In Stock
5 mg	$64	In Stock	In Stock
10 mg	$108	In Stock	In Stock
25 mg	$222	In Stock	In Stock
50 mg	$355	In Stock	In Stock
100 mg	$573	In Stock	In Stock
200 mg	$779	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$67	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.9%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	KY1220 destabilizes both β-catenin and Ras by targeting the Wnt/β-catenin pathway. It has an IC50 of 2.1 μM in HEK293 reporter cells.
Targets&IC50	Wnt/β-catenin:2.1 μM
In vitro	KY1220 accelerates the degradation rates of both β-catenin and Ras in SW480 cell lines. KY1220 dose-dependently reduces Wnt3a-CM-induced TOPflash reporter activation and mRNA expression of Wnt target genes CCND1 and MYC in HEK293 cells. Ras destabilization by KY1220 consequently inhibits the activities of both ERK and Akt, which are downstream effectors of Ras in SW480 cells harboring a KRAS mutation. In HEK293 cells, both β-catenin and pan-Ras protein levels are similarly reduced in a dose-dependent manner after treatment with KY1220, whereas the mRNA levels of CTNNB1 (which encodes β-catenin), NRAS, KRAS, and HRAS remain unchanged. K-Ras, which has a critical role in the progression of CRCs, is also destabilized by KY1220 via polyubiquitin-dependent proteasomal degradation. The proliferation and transformation of the HCT15, SW480, D-WT, and D-MT CRC cells are efficiently inhibited after treatment with KY1220.

Chemical Properties

Molecular Weight	314.32
Formula	C₁₄H₁₀N₄O₃S
Cas No.	292168-79-7
Smiles	[O-][N+](=O)c1ccc(cc1)-n1cccc1\C=C1/NC(=S)NC1=O
Relative Density.	1.54 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (318.15 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1815 mL	15.9074 mL	31.8147 mL	159.0736 mL
5 mM	0.6363 mL	3.1815 mL	6.3629 mL	31.8147 mL
10 mM	0.3181 mL	1.5907 mL	3.1815 mL	15.9074 mL
20 mM	0.1591 mL	0.7954 mL	1.5907 mL	7.9537 mL
50 mM	0.0636 mL	0.3181 mL	0.6363 mL	3.1815 mL
100 mM	0.0318 mL	0.1591 mL	0.3181 mL	1.5907 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc