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iCRT3

Name: iCRT3
Brand: TargetMol
SKU: T4302
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4302Cas No. 901751-47-1

iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor.

iCRT3

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Purity: 98.47%

Catalog No. T4302Cas No. 901751-47-1

iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$38	In Stock	In Stock
10 mg	$58	In Stock	In Stock
25 mg	$112	In Stock	In Stock
50 mg	$176	In Stock	In Stock
100 mg	$287	-	In Stock
200 mg	$397	-	In Stock
1 mL x 10 mM (in DMSO)	$42	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

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Purity:98.47%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor.
Targets&IC50	Wnt/β-catenin:8.2 nM
In vitro	Treatment with iCRT3 at doses of 12.5, 25, 50, and 75 μM decreases TNF-α levels by 14.7%, 18.5%, 44.9% and 61.3%, respectively.
In vivo	The IL-6 levels in the 10?mg/kg iCRT3 treatment mice are lower.
Kinase Assay	In the determination of full-length baculovirus expressed Syk kinase activity, the reaction system was 25 μL containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 2 mM DTT, 0.1 mM Na3VO4, 10 mM MgCl2, 0.5 μM Promega PTK Biotin Peptide Substrate 1, 0.01% casein, 1, 0.01% Triton X-100, 0.25% Glycerol, and 40 mM ATP (Km for ATP). After incubation for 60 minutes at room temperature, the reaction was stopped by adding 30 mM EDTA (30 μL of SA-APC and 4 nM PT-66 antibody). After measuring the plate, the IC50 value of the test compound was calculated using a 4-parameter linear regression algorithm.
Cell Research	Cells are seeded into 96-well plates to a density of 5×103 cells per well and incubated in the culture medium with iCRT3 for an additional 48 h. Cell viability and cell apoptosis assays are carried out using a Cell Counting kit-8 and a Caspase-Glo 3/7 assay kit according to the manufacturer's instructions, respectively.
Animal Research	NOD-SCID BALB/c mice inoculated A172 cells are treated with iCRT3 (5 mg/kg) diluted in PBS i.p. triweekly.

Chemical Properties

Molecular Weight	394.53
Formula	C₂₃H₂₆N₂O₂S
Cas No.	901751-47-1
Smiles	CCc1ccc(cc1)-c1nc(CSCC(=O)NCCc2ccccc2)c(C)o1
Relative Density.	1.150 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (126.73 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.07 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5347 mL	12.6733 mL	25.3466 mL	126.7331 mL
5 mM	0.5069 mL	2.5347 mL	5.0693 mL	25.3466 mL
10 mM	0.2535 mL	1.2673 mL	2.5347 mL	12.6733 mL
20 mM	0.1267 mL	0.6337 mL	1.2673 mL	6.3367 mL
50 mM	0.0507 mL	0.2535 mL	0.5069 mL	2.5347 mL
100 mM	0.0253 mL	0.1267 mL	0.2535 mL	1.2673 mL

Calculator

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Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc