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iCRT3

Catalog No. T4302 CAS 901751-47-1

iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

iCRT3, CAS 901751-47-1

Pack Size	Availability	Price/USD
2 mg	In stock	$ 39.00
5 mg	In stock	$ 64.00
10 mg	In stock	$ 97.00
25 mg	In stock	$ 187.00
50 mg	In stock	$ 293.00
100 mg	In stock	$ 478.00
500 mg	In stock	$ 1,050.00
1 mL * 10 mM (in DMSO)	In stock	$ 70.00

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Purity: 98.32%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	iCRT3 is a Wnt and β-catenin-responsive transcription inhibitor.
Targets&IC50	Wnt/β-catenin:8.2 nM
In vitro	Treatment with iCRT3 at doses of 12.5, 25, 50, and 75 μM decreases TNF-α levels by 14.7%, 18.5%, 44.9% and 61.3%, respectively.
In vivo	The IL-6 levels in the 10?mg/kg iCRT3 treatment mice are lower.
Kinase Assay	In the determination of full-length baculovirus expressed Syk kinase activity, the reaction system was 25 μL containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 2 mM DTT, 0.1 mM Na3VO4, 10 mM MgCl2, 0.5 μM Promega PTK Biotin Peptide Substrate 1, 0.01% casein, 1, 0.01% Triton X-100, 0.25% Glycerol, and 40 mM ATP (Km for ATP). After incubation for 60 minutes at room temperature, the reaction was stopped by adding 30 mM EDTA (30 μL of SA-APC and 4 nM PT-66 antibody). After measuring the plate, the IC50 value of the test compound was calculated using a 4-parameter linear regression algorithm.
Cell Research	Cells are seeded into 96-well plates to a density of 5×103 cells per well and incubated in the culture medium with iCRT3 for an additional 48 h. Cell viability and cell apoptosis assays are carried out using a Cell Counting kit-8 and a Caspase-Glo 3/7 assay kit according to the manufacturer's instructions, respectively.
Animal Research	NOD-SCID BALB/c mice inoculated A172 cells are treated with iCRT3 (5 mg/kg) diluted in PBS i.p. triweekly.

Molecular Weight	394.53
Formula	C23H26N2O2S
CAS No.	901751-47-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 150 mg/mL

References and Literature

1. Xiao H, et al. A Novel Positive Feedback Loop Between NTSR1 and Wnt/β-Catenin Contributes to Tumor Growth of Glioblastoma. Cell Physiol Biochem. 2017 Oct 24;43(5):2133-2142. 2. Chatterjee SS, et al. Inhibition of β-catenin-TCF1 interaction delays differentiation of mouse embryonic stem cells. J Cell Biol. 2015 Oct 12;211(1):39-51. 3. Sharma A, et al. Mitigation of sepsis-induced inflammatory responses and organ injury through targeting Wnt/β-catenin signaling. doi: 10.1038/s41598-2017-08711-6. 4. Wang J, Gong L, Zhang W, et al. Wnt/β-catenin signaling pathway inhibits porcine reproductive and respiratory syndrome virus replication by enhancing the nuclear factor-κB-dependent innate immune response[J]. Veterinary Microbiology . 2020: 108904.

Citations

1. Wang J, Gong L, Zhang W, et al. Wnt/β-catenin signaling pathway inhibits porcine reproductive and respiratory syndrome virus replication by enhancing the nuclear factor-κB-dependent innate immune response. Veterinary Microbiology. 2020: 108904.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compounds Library Max Bioactive Compound Library Neural Regeneration Compound Library Apoptosis Compound Library Anti-Cancer Compound Library Osteogenesis Compound Library Stem Cell Differentiation Compound Library Anti-Fibrosis Compound Library Anti-COVID-19 Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

iCRT3 901751-47-1 Apoptosis Cytoskeletal Signaling Stem Cells Wnt/beta-catenin Inhibitor Wnt inhibit iCRT-3 iCRT 3 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.