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Hematein

Name: Hematein
Brand: TargetMol
SKU: T15469
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15469Cas No. 475-25-2

Alias Hydroxybrasilein

Hematein inhibits Akt/PKB Ser129 phosphorylation, the Wnt/TCF pathway, and increases apoptosis in lung cancer cells. Hematein is an oxidation product of hematoxylin acted as a dye and it also is an allosteric casein kinase II inhibitor (IC50: 0.74 μM).

Hematein

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Catalog No. T15469Alias HydroxybrasileinCas No. 475-25-2

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 mg	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:Black

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Product Introduction

Bioactivity

Description	Hematein inhibits Akt/PKB Ser129 phosphorylation, the Wnt/TCF pathway, and increases apoptosis in lung cancer cells. Hematein is an oxidation product of hematoxylin acted as a dye and it also is an allosteric casein kinase II inhibitor (IC50: 0.74 μM).
Targets&IC50	CK2:0.74 μM
In vitro	Hematein (50 and 100 μM; 48 hours) inhibits CK2-specific Akt phosphorylation. Hematein (50 and 100 μM; 48 hours) causes apoptosis in A427 lung cancer cells. Hematein (10-100 μM; 14 days) inhibits cell growth in A427 lung cancer cells [1].
In vivo	In A427 lung cancer cell xenografts, Hematein (i.p.; 50 mg/kg; twice a week for 6 weeks) inhibits tumor growth [1].
Synonyms	Hydroxybrasilein

Chemical Properties

Molecular Weight	300.26
Formula	C₁₆H₁₂O₆
Cas No.	475-25-2
Smiles	OC1=CC2=C3c4ccc(O)c(O)c4OCC3(O)CC2=CC1=O
Relative Density.	1.77g/cm3

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 5.5 mg/mL (18.32 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (3.33 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3304 mL	16.6522 mL	33.3045 mL	166.5223 mL
5 mM	0.6661 mL	3.3304 mL	6.6609 mL	33.3045 mL
10 mM	0.3330 mL	1.6652 mL	3.3304 mL	16.6522 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc