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H-89

Name: H-89
Brand: TargetMol
SKU: T11524
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11524Cas No. 127243-85-0

Alias Protein kinase inhibitor H-89

H-89 is a selective and potent protein kinase A inhibitor (IC50: 48 nM), a candidate cardioprotectant, induces spatial learning impairment in rats, and can be used to study myocardial infarction.

H-89

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Purity: 98.06%

Catalog No. T11524Alias Protein kinase inhibitor H-89Cas No. 127243-85-0

H-89 is a selective and potent protein kinase A inhibitor (IC50: 48 nM), a candidate cardioprotectant, induces spatial learning impairment in rats, and can be used to study myocardial infarction.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$34	In Stock	In Stock
10 mg	$54	In Stock	In Stock
25 mg	$116	In Stock	In Stock
50 mg	$178	In Stock	In Stock
100 mg	$267	-	In Stock
1 mL x 10 mM (in DMSO)	$37	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:98.06%

Appearance:Solid

Color:White

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	H-89 is a selective and potent protein kinase A inhibitor (IC50: 48 nM), a candidate cardioprotectant, induces spatial learning impairment in rats, and can be used to study myocardial infarction.
Targets&IC50	protein kinase A:48 nM
In vitro	Pretreatment of PC12D cells with H-89 (30 μM) significantly inhibited cAMP-dependent histone IIb phosphorylation activity in cell lysates, but did not affect other protein phosphorylation activities [1]; in rat skin EDL fibers , H-89 (10 μM) only slightly inhibited the force response to depolarization; H-89 (1-2 μM) significantly slowed the re-excitation rate in rat skin EDL fibers, most likely because of its Deleteriously affects the T system potential; H-89 (10-100 μM) also inhibits the net uptake of Ca2+ by SR. [2]
In vivo	b>METHODS: H-89 (0.05, 0.1, 0.2 mg/100 g, intraperitoneal injection) was used 30 minutes before the infusion of pentylene tetrazolium (PTZ) in mice to study the effect of H-89 on Bucladesine-induced epileptic activity in PTZ-treated mice. RESULTS: H-89 (0.2 mg/100 g) significantly increased the seizure latency and threshold of PTZ-treated animals; H-89 (0.05, 0.2 mg/100 g) blocked the epileptogenic activity of Bucladesine and significantly increased the seizure latency and seizure threshold. [3]
Synonyms	Protein kinase inhibitor H-89

Chemical Properties

Molecular Weight	446.36
Formula	C₂₀H₂₀BrN₃O₂S
Cas No.	127243-85-0
Smiles	O=S(=O)(NCCNCC=CC1=CC=C(Br)C=C1)C2=CC=CC=3C=NC=CC32
Relative Density.	1.436 g/cm3

Storage & Solubility Information

Storage

store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 72 mg/mL (161.3 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.48 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2403 mL	11.2017 mL	22.4034 mL	112.0172 mL
5 mM	0.4481 mL	2.2403 mL	4.4807 mL	22.4034 mL
10 mM	0.2240 mL	1.1202 mL	2.2403 mL	11.2017 mL
20 mM	0.1120 mL	0.5601 mL	1.1202 mL	5.6009 mL
50 mM	0.0448 mL	0.2240 mL	0.4481 mL	2.2403 mL
100 mM	0.0224 mL	0.1120 mL	0.2240 mL	1.1202 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc