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FMK 9a

Name: FMK 9a
Brand: TargetMol
SKU: T15303
Price: 113 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15303Cas No. 1955550-51-2

FMK 9a is an irreversible inhibitor of ATG4B with IC50 values of 80 and 73 nM in the TR-FRET and cellular-based LRA assays.

FMK 9a

🥰Excellent

Purity: 98.97%

Catalog No. T15303Cas No. 1955550-51-2

FMK 9a is an irreversible inhibitor of ATG4B with IC50 values of 80 and 73 nM in the TR-FRET and cellular-based LRA assays.

Pack Size	Price	Availability
1 mg	$113	In Stock
5 mg	$289	In Stock
10 mg	$428	In Stock
25 mg	$689	In Stock
50 mg	$977	In Stock
100 mg	$1,280	In Stock
200 mg	$1,760	In Stock
1 mL x 10 mM (in DMSO)	$298	In Stock

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Bulk & Custom

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Purity:98.97%

ee:100%

Resource Download

COA CSFC FNMR LCMS HNMR

Product Introduction

Bioactivity

Description	FMK 9a is an irreversible inhibitor of ATG4B with IC50 values of 80 and 73 nM in the TR-FRET and cellular-based LRA assays.
Targets&IC50	Autophagin-1 (FRET assay):80 nM, Autophagin-1 (LRA assay):73 nM
In vitro	FMK 9a forms a covalent bond with the more reactive thiol group of Cys74 located at the catalytic site of ATG4B thereby inactivating ATG4B proteolytic activity[1].
In vivo	FMK 9a shows moderate solubility and high liver microsome clearances for human and mice of 13.9 and 70 mL/kg/min[1].

Chemical Properties

Molecular Weight	392.42
Formula	C₂₃H₂₁FN₂O₃
Cas No.	1955550-51-2
Smiles	FCC(=O)CNC(=O)[C@H](Cc1ccccc1)NC(=O)c1cccc2ccccc12
Relative Density.	1.241 g/cm3 (Predicted)
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 95 mg/mL (242.09 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (8.41 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5483 mL	12.7415 mL	25.4829 mL	127.4145 mL
5 mM	0.5097 mL	2.5483 mL	5.0966 mL	25.4829 mL
10 mM	0.2548 mL	1.2741 mL	2.5483 mL	12.7415 mL
20 mM	0.1274 mL	0.6371 mL	1.2741 mL	6.3707 mL
50 mM	0.0510 mL	0.2548 mL	0.5097 mL	2.5483 mL
100 mM	0.0255 mL	0.1274 mL	0.2548 mL	1.2741 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc