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Bohemine

Name: Bohemine
Brand: TargetMol
SKU: T2029
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2029Cas No. 189232-42-6

Bohemine is a cyclin-dependent kinase inhibitor.

Bohemine

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Purity: 99.53%

Catalog No. T2029Cas No. 189232-42-6

Bohemine is a cyclin-dependent kinase inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$35	In Stock	In Stock
25 mg	$71	In Stock	In Stock
50 mg	$108	In Stock	In Stock
100 mg	$163	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.53%

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bohemine AI Summary

Bohemine is a multi-target inhibitor with notable bioactivities across various assays and biological systems. It inhibits Cyclin-dependent kinase 1 (CDK1) with an IC50 of 2500 nM and Cyclin-dependent kinase 2 (CDK2) with an IC50 of 800 nM. Additionally, it inhibits recombinant CDK1-cyclin B with an IC50 of 1100 nM and demonstrates broad in vitro cytotoxic effects on several cancer cell lines, including MCF-7, K562, CEM, HOS, and G361 with IC50 values of 28000 nM, 113000 nM, 27000 nM, 58000 nM, and 45000 nM, respectively. Bohemine shows further kinase inhibition with an IC50 of 1000 nM and induces thermal stabilization across multiple protein kinases such as AMPKA2, CAMKs, CK1 family members, PIMs, PLKs, RSKs, SLK, MPSK1, TNIK, PBK, and YSK1, suggesting broad-spectrum kinase interaction. Apart from kinase inhibition, Bohemine inhibits Tyrosyl-DNA Phosphodiesterase (TDP1) and GCN5L2 with potencies of 35481.3 nM and 31622.8 nM, respectively, and blocks the interaction between CBF-beta and RUNX1, showing potential for treating Acute Myeloid Leukemia with a potency of 31622.8 nM. The compound also exhibits agonistic activity at N-type Cav2.2 channels in tsA201 cells, although with an EC50 value greater than 1000000 nM, implying weak activity in this context. In cellular assays, Bohemine displays cytotoxicity against human BJ cells at 30 µM with 119.8% activity relative to control after 72 hours and shows leishmanicidal activity against Leishmania turanica promastigotes with an activity of 55.4% at the same concentration and time point. Additionally, it inhibits Leishmania CRK3/CYC6 using histone H1/[gamma33-P]ATP as a substrate at 30 µM, with a 49.2% inhibition rate after 30 minutes compared to the control..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Bohemine is a cyclin-dependent kinase inhibitor.
Targets&IC50	CDK2-CyclinA:83 μM (IC50), ERK2:52 μM (IC50), CDK2-CyclinE:4.6 μM (IC50), CDK9-CyclinT1:2.7 μM (IC50)

Chemical Properties

Molecular Weight	340.42
Formula	C₁₈H₂₄N₆O
Cas No.	189232-42-6
Smiles	CC(C)n1cnc2c(NCc3ccccc3)nc(NCCCO)nc12
Relative Density.	1.27 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (146.88 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.88 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9375 mL	14.6877 mL	29.3755 mL	146.8774 mL
5 mM	0.5875 mL	2.9375 mL	5.8751 mL	29.3755 mL
10 mM	0.2938 mL	1.4688 mL	2.9375 mL	14.6877 mL
20 mM	0.1469 mL	0.7344 mL	1.4688 mL	7.3439 mL
50 mM	0.0588 mL	0.2938 mL	0.5875 mL	2.9375 mL
100 mM	0.0294 mL	0.1469 mL	0.2938 mL	1.4688 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc