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Amifampridine

Name: Amifampridine
Brand: TargetMol
SKU: T4497
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4497Cas No. 54-96-6

Alias Pyridine-3,4-Diamine, 3,4-Pyridinediamine, 3,4-Diaminopyridine

Amifampridine (3,4-Diaminopyridine) is primarily used to treat rare muscle diseases. It blocks potassium channel efflux in nerve terminals, increasing action potential duration, which allows Ca2+ channels to remain open longer and enhances acetylcholine release to stimulate muscles at the end plate.

Amifampridine

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Purity: 99.82%

Catalog No. T4497Alias Pyridine-3,4-Diamine, 3,4-Pyridinediamine, 3,4-DiaminopyridineCas No. 54-96-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.82%

Appearance:Solid

Color:Red

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COA HNMR LCMS

Product Introduction

Amifampridine AI Summary

Amifampridine exhibits a diverse range of bioactivities. Primarily, the compound selectively increases both basal and K+-stimulated acetylcholine (AcCh) release across various brain regions, including the hippocampus, cortex, and striatum, with dose-dependent effects in these areas. Additionally, it inhibits [3H]choline uptake at choline transporter 1 in mouse brain synaptosomes with a Ki value of 820351.54 nM. Beyond its impact on neurotransmitter release, Amifampridine demonstrates inhibitory activities against multiple targets such as lipid storage, AmpC beta-lactamase, histone lysine methyltransferase G9a, and several others, with potencies in the nanomolar to micromolar range. The compound also shows antiviral properties, including inhibition of cytotoxicity induced by SARS-CoV-2 in various cell lines and inhibition of the SARS-CoV-2 3CL-Pro protease. However, its effectiveness against SARS-CoV-2 is limited, with an IC50 value greater than 20000.0 nM and variable inhibition percentages. Additionally, the compound exhibits notable activity in an enzymatic assay of human HDAC6 and functions as a substrate for human OCT1, characterized by specific kinetic parameters and intrinsic clearance values. Despite these activities, Amifampridine has shown no significant bioactivity in binding affinity and inhibition assays against several human and rat receptors, enzymes, and ion channels, with all AC50 values exceeding 30,000.0 nM..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Amifampridine (3,4-Diaminopyridine) is primarily used to treat rare muscle diseases. It blocks potassium channel efflux in nerve terminals, increasing action potential duration, which allows Ca2+ channels to remain open longer and enhances acetylcholine release to stimulate muscles at the end plate.
Synonyms	Pyridine-3,4-Diamine, 3,4-Pyridinediamine, 3,4-Diaminopyridine

Chemical Properties

Molecular Weight	109.13
Formula	C₅H₇N₃
Cas No.	54-96-6
Smiles	NC1=C(N)C=NC=C1
Relative Density.	1.251g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 24 mg/mL (219.92 mM), Sonication is recommended.

DMSO: 60 mg/mL (549.8 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (18.33 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	9.1634 mL	45.8169 mL	91.6338 mL	458.1692 mL
5 mM	1.8327 mL	9.1634 mL	18.3268 mL	91.6338 mL
10 mM	0.9163 mL	4.5817 mL	9.1634 mL	45.8169 mL
20 mM	0.4582 mL	2.2908 mL	4.5817 mL	22.9085 mL
50 mM	0.1833 mL	0.9163 mL	1.8327 mL	9.1634 mL
100 mM	0.0916 mL	0.4582 mL	0.9163 mL	4.5817 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc