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Ac-DEVD-AMC

Name: Ac-DEVD-AMC
Brand: TargetMol
SKU: T18909
Price: 43 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18909Cas No. 169332-61-0

Alias AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN

Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 .

Ac-DEVD-AMC

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Purity: 98.20%

Catalog No. T18909Alias AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARINCas No. 169332-61-0

Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 .

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$43	In Stock	In Stock
5 mg	$126	In Stock	In Stock
10 mg	$198	In Stock	In Stock
25 mg	$347	In Stock	In Stock
50 mg	$516	In Stock	In Stock
100 mg	$736	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:98.20%

Appearance:Solid

Color:White

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COA MS HPLC

Product Introduction

Bioactivity

Description	Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 .
In vitro	The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2].
Cell Research	Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2].
Synonyms	AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN

Chemical Properties

Molecular Weight	675.64
Formula	C₃₀H₃₇N₅O₁₃
Cas No.	169332-61-0
Smiles	CC(C)[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(C)=O)C(=O)N[C@@H](CC(O)=O)C(=O)Nc1ccc2c(C)cc(=O)oc2c1
Relative Density.	1.423 g/cm3
Sequence	Ac-Asp-Glu-Val-Asp-AMC
Sequence Short	DEVD-AMC

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: ≤ 10 mg/mL (14.80 mM), Sonication is recommended.

H2O: < 0.1 mg/mL (insoluble)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4801 mL	7.4004 mL	14.8008 mL	74.0039 mL
5 mM	0.2960 mL	1.4801 mL	2.9602 mL	14.8008 mL
10 mM	0.1480 mL	0.7400 mL	1.4801 mL	7.4004 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc