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ZD7288

Catalog No. T7516 CAS 133059-99-1

Synonyms: ICI D7288, N-乙基-1,6-二氢-1,2-二甲基-6-(甲基亚氨基)-N-苯基-4-嘧啶胺盐酸盐

ZD7288, a selective hyperpolarization-activated cyclic nucleotide-gated channel blocker, inhibits hippocampal synaptic plasticity.

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ZD7288, CAS 133059-99-1

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Purity: 98%

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Description	ZD7288, a selective hyperpolarization-activated cyclic nucleotide-gated channel blocker, inhibits hippocampal synaptic plasticity.
In vitro	ZD7288 attenuated glutamate-induced rises in [Ca(2+)]i in a concentration-dependent manner and reversed 8-Br-cAMP-mediated facilitation of these glutamate-induced [Ca(2+)]i rises.?ZD7288 inhibits hippocampal synaptic plasticity both glutamate release and resultant [Ca(2+)]i increases in rat hippocampal neurons[1].
Cell Research	Cultured hippocampal neurons were incubated with 1 μM Fura-2 acetoxy-methylol ester for 30 minutes at 37°C, washed three times with artificial cerebrospinal fluid (containing 140 mM NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM glucose and 10 mM hydroxyethyl piperazine ethanesulfonic acid, pH 7.3), and then incubated at room temperature in the dark for 30 minutes.?Fura-2 fluorescence was observed by a Ratio Vision digital fluorescence microscopy system.?Fluorescence signals were evoked by 340 and 380 nm excitation wavelengths and collected at 510 nm by TILLvisION 4.0 software.?The 340:380 nm fluorescence ratio was used to represent [Ca2+]i.?Peak calcium change was represented as the percentage increase from baseline.?Neurons were incubated in ZD7288 (25, 50 or 100 μM) or 8-Br-cAMP (5 or 50 μM) for 15 minutes prior to stimulation with 50 μM glutamate.?All experiments were repeated in triplicate, using different batches of cells across 4–5 dishes[1].

Synonyms	ICI D7288, N-乙基-1,6-二氢-1,2-二甲基-6-(甲基亚氨基)-N-苯基-4-嘧啶胺盐酸盐
Molecular Weight	292.81
Formula	C15H21ClN4
CAS No.	133059-99-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 50 mg/mL (170.76 mM)

H2O: 50 mg/mL (170.76 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Zhang X X , Min X C , Xu X L , et al. ZD7288, a selective hyperpolarization-activated cyclic nucleotide-gated channel blocker, inhibits hippocampal synaptic plasticity[J]. 2016, 011(005):779-786. 2. Pirtle T J , Carr T L , Khurana T , et al. ZD7288 and mibefradil inhibit the myogenic heartbeat in, Daphnia magna, indicating its dependency on HCN and T-type calcium ion channels[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2018, 222:36-42.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cardiovascular Disease Compound Library Inhibitor Library Ion Channel Inhibitor Library Bioactive Compound Library

Related Products

Related compounds with same targets

ZD7288 Zatebradine hydrochloride

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Keywords

ZD7288 133059-99-1 离子通道 HCN Channel inhibit ICI D-7288 Hyperpolarization activated cyclic nucleotide gated channels ICI D7288 ICI D 7288 ZD 7288 Inhibitor ZD-7288 inhibitor

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