store at low temperture,keep away from direct sunlight
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
GKT137831 is a potent, specific dual NADPH oxidase (NOX1/4) inhibitor.
Description | GKT137831 is a potent, specific dual NADPH oxidase (NOX1/4) inhibitor. |
Targets&IC50 | NOX4:140 nM(Ki), NOX1:110 nM(Ki) |
Kinase Assay | Kinase Assay: IC50 of tyrosine kinase activity is measured by an enzyme-linked immunosorbent assay (ELISA) with recombinant catalytic domains of a panel of receptor and non-receptor tyrosine kinases (in some cases only part of the catalytic domain is used). Saracatinib dose ranges from 0.001-10 mM. Speci?city assays against a panel of serine/threonine kinases are performed using a ?lter capture assay with 32P. Brie?y, multidrop 384 plates containing 0.5 μL Saracatinib or controls (DMSO) alone or pH 3.0 buffer controls) are incubated with 15 μL of enzyme plus peptide/protein substrate for 5 min before the reaction is initiated by the addition of 10 μL of 20 mM Mg-ATP. For all enzymes the ?nal concentration is approximated to the Michaelis constant (Km). Assays are carried out for 30min at room temperature before termination by the addition of 5 μL orthophosphoric acid. After mixing, the well contents are harvested onto a P81 Uni?lter plate, using orthophosphoric acid as the wash buffer. Then IC50 is calculated. |
Cell Research | Hypoxic HPASMC and HPAEC proliferation is determined using MTT assay, by Western blotting to detect proliferating cell nuclear antigen (PCNA) expression, or by manual cell counting after Trypan blue staining. Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit is used to measure Water2 released into the culture media from HPAECs or HPASMCs. After exposure to control or hypoxic environments for 72 hours, Amplex Red reagent is added, and the cells are returned to control or hypoxic environments for an additional hour before fluorescence measurements. (Only for Reference) |
Synonyms | GKT137831 |
Molecular Weight | 394.85 |
Formula | C21H19ClN4O2 |
CAS No. | 1218942-37-0 |
store at low temperture,keep away from direct sunlight
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 72 mg/mL (182.3 mM)
Ethanol: <1 mg/mL
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Setanaxib 1218942-37-0 Apoptosis Cell Cycle/Checkpoint Immunology/Inflammation Metabolism NADPH-oxidase Ferroptosis NADPH NOX GKT-137831 GKT 137831 GKT-831 GKT831 inhibit GKT 831 Inhibitor GKT137831 NADPH Oxidase inhibitor