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SP2509

Catalog No. T2304 CAS 1423715-09-6

Synonyms: HCI-2509

SP2509 is a specific histone demethylase LSD1 inhibitor(IC50 =13 nM).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

SP2509, CAS 1423715-09-6

Pack Size	Availability	Price/USD
5 mg	In stock	$ 52.00
10 mg	In stock	$ 79.00
25 mg	In stock	$ 143.00
50 mg	In stock	$ 243.00
100 mg	In stock	$ 397.00
500 mg	In stock	$ 936.00
1 mL * 10 mM (in DMSO)	In stock	$ 54.00

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Purity: 99.53%

Purity: 99.49%

Purity: 98.21%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	SP2509 is a specific histone demethylase LSD1 inhibitor(IC50 =13 nM).
Targets&IC50	LSD1:13 nM
Kinase Assay	SP2509 activity assays: Test compounds are diluted to 20 × the desired test concentration in 100% DMSO and 2.5?μL of the diluted drug sampleis added to a black 384-well plate. The LSD1 enzyme stock is diluted 17-fold with assay buffer and 40?μL of the diluted LSD1 enzyme is added to the appropriate wells. Substrate, consisting of horseradish peroxidase, dimethyl K4 peptide corresponding to the first 21 amino acids of the N-terminal tail of histone H3, and 10-acetyl-3,7-dihydroxyphenoxazine is then added to wells. Resorufin is analyzed on an Envision plate reader with an excitation wavelength of 530?nm and an emission wavelength of 595?nm. The activity of SP2509 on the other oxidases is determined by using commercially available kits. The glucose oxidase activity (which also noncovalently binds FAD in an elongate conformation), is determined using the glucose oxidase kit. The MAO assays are performed using the MAO-glo kit with MAO-A and MAO-B.
Cell Research	Cultured AML cells are treated with SP2509 and/or PS for 96?h. At the end of treatment, cells are washed free of the drugs and 500 cells per condition are plated in methylcellulose and incubated at 37?°C. Colony formation is measured 7–10 days after plating.(Only for Reference)

Synonyms	HCI-2509
Molecular Weight	437.9
Formula	C19H20ClN3O5S
CAS No.	1423715-09-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 36 mg/mL (82.2 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Fiskus W, et al. Leukemia. 2014, 28(11), 2155-2164.

Related compound libraries

This product is contained In the following compound libraries：

Highly Selective Inhibitor Library Inhibitor Library Bioactive Compounds Library Max Bioactive Compound Library Apoptosis Compound Library Autophagy Compound Library Anti-Aging Compound Library Histone Modification Compound Library Epigenetics Compound Library Chromatin Modification Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SP2509 1423715-09-6 Apoptosis Chromatin/Epigenetic Histone Demethylase HCI 2509 SP 2509 SP-2509 inhibit Inhibitor HCI-2509 HCI2509 inhibitor

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