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DNA Damage/DNA Repair DNA RU320521

RU320521

Catalog No. T5486   CAS 2262452-06-0
Synonyms: RU.521, RU.521

RU.521 (RU320521) is a potent dsDNA inhibitor (IC50 : 700 nM), inhibition of the dsDNA pathway by RU.521 requires the presence of cGAS.

RU320521, CAS 2262452-06-0
Pack Size Availability Price/USD Quantity
2 mg In stock 84.00
5 mg In stock 120.00
10 mg In stock 213.00
25 mg In stock 487.00
50 mg In stock 842.00
100 mg In stock 1302.00
1 mL * 10 mM (in DMSO) In stock 133.00
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Purity 98.00%
Biological Description
Chemical Properties
Storage & Solubility Information
Description RU.521 (RU320521) is a potent dsDNA inhibitor (IC50 : 700 nM), inhibition of the dsDNA pathway by RU.521 requires the presence of cGAS.
Targets&IC50 dsDNA : ic50 700 nM,  
In vivo Treatment of primary BMDMs with RU.521 or its analogs reduced IFNB1 expression in mice, indicating their effectiveness in suppressing intrinsic DNA-dependent, constitutively-activated type I interferon expression in cells deficient of a cytoplasmic DNA exonuclease.
Cell Research
Small-molecule compounds were serially diluted to concentrations spanning the range tested in the response curves were added to 6.7 × 10^5 RAW-Blue macrophages plated 16h prior in 96-well dishes, then harvested 72h after compound addition. ATP was measured using CellTiter Glo Viability Assay using 50 μM Tamoxifen as a positive control for cytotoxicity. Viability values were generated using vehicle (DMSO) or the first dose as 100% and Tamoxifen as 0%. Outliers were removed.
Animal Research
the chronically elevated levels of cytokines observed in Trex1 null mice are a consequence of constitutively activated cGAS, due to the inability to eliminate aberrantly localized self-DNA. We harvested BMDMs from 6–8-week old Trex1 -/- mice, treated them with each compound, and measured expression levels of IFNB1 by quantitative reverse transcription PCR (qRT-PCR). Treatment of primary BMDMs with RU.521 or its analogs reduced IFNB1 expression, indicating their effectiveness in suppressing intrinsic DNA-dependent, constitutively-activated type I interferon expression in cells deficient of a cytoplasmic DNA exonuclease.
Synonyms RU.521 , RU.521
Purity 98.00%
Molecular Weight 414.22
Formula C19H11Cl2N4O3
CAS No. 2262452-06-0

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 125 mg/mL (301.04 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Vincent J, et al. Small molecule inhibition of cGAS reduces interferon expression in primary macrophages from autoimmune mice. Nat Commun. 2017 Sep 29;8(1):750.

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