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Pyranine

Catalog No. T16689 CAS 6358-69-6

Synonyms: HPTS, Solvent Green 7

Pyranine (Solvent Green 7) is reported as a new class of fluorescent chemosensor for the Cu+ ion.

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Pyranine, CAS 6358-69-6

Pack Size	Availability	Price/USD
500 mg	In stock	$ 46.00
1 g	In stock	$ 79.00
1 mL * 10 mM (in DMSO)	In stock	$ 51.00

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Purity: 100%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Pyranine (Solvent Green 7) is reported as a new class of fluorescent chemosensor for the Cu+ ion.

In vitro

Pyranine (Py) is a fluorescence probe[3] and is capable of discriminating ranges of cations from the Cu+ ion, even in competing environment. Pyranine displays a rapid fluorescence response (t1/2=1.66 min) towards the Cu+ ion, and the micromolar detection limit enables the detection of the ion in environmental samples. The observed stoichiometry of complexation between Pyranine and Cu+ is 2:1[1]. The pH is calculated by measuring the ratio of fluorescent intensity at two excitation wavelengths, I463/I404, which is dependent on pH but independent of the concentration of the fluorophore and other variables which can alter the intensity. In the rabbit eye, Pyranine in the cornea and anterior chamber is observed to undergo easily measurable changes in fluorescent ratios associated with lid closure and contact lens wear, indicating its sensitivity to mild changes in pH[2].

Synonyms	HPTS, Solvent Green 7
Molecular Weight	524.39
Formula	C16H7Na3O10S3
CAS No.	6358-69-6

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 100 mg/mL (190.70 mM), Sonication is recommended.

DMSO: 6.88 mg/mL (13.11 mM), Sonication is recommended.

References and Literature

1. Saha T, et al. In vitro sensing of Cu(+) through a green fluorescence rise of pyranine. Photochem Photobiol Sci. 2014 Oct;13(10):1427-33. 2. Thomas JV, et al. The fluorescent indicator pyranine is suitable for measuring stromal and cameral pH in vivo. Exp Eye Res. 1990 Mar;50(3):241-9. 3. Evingür GA, et al. Kinetic models for the dynamical behavior of polyacrylamide (PAAm)-κ-carrageenan (κC) composite gels. J Biol Phys. 2015 Jan;41(1):37-47. 4. Ge S, Wang X, Hou Y, et al. Repositioning of histamine H1 receptor antagonist: Doxepin inhibits viropexis of SARS-CoV-2 Spike pseudovirus by blocking ACE2[J]. European Journal of Pharmacology. 2021: 173897.

Citations

1. Yu, W., et al. AMPK-SIRT1 Pathway Modulates The Apoptosis, Proliferation and Migration of AR42J Cells By Regulating p53 and NF-κB. European Journal of Pharmacology. 2021 2. Ge S, Wang X, Hou Y, et al. Repositioning of histamine H1 receptor antagonist: Doxepin inhibits viropexis of SARS-CoV-2 Spike pseudovirus by blocking ACE2. European Journal of Pharmacology. 2021 Apr 5;896:173897. doi: 10.1016/j.ejphar.2021.173897. Epub 2021 Jan 23.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Pyranine 6358-69-6 Others Solvent Green-7 inhibit fluorescent Solvent Green7 indicator Cu+ Inhibitor HPTS Solvent Green 7 inhibitor

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