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Procaine

Catalog No. T0029 CAS 59-46-1

Synonyms: Duracaine, Spinocaine, Novocaine, Vitamin H3

Procaine (Vitamin H3) is a local anesthetic of the ester type that has a slow onset and a short duration of action. It is mainly used for infiltration anesthesia, peripheral nerve block, and spinal block.

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Procaine, CAS 59-46-1

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500 mg	In stock	$ 46.00
1 g	Inquiry	$ 53.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.55%

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Description	Procaine (Vitamin H3) is a local anesthetic of the ester type that has a slow onset and a short duration of action. It is mainly used for infiltration anesthesia, peripheral nerve block, and spinal block.
In vitro	The viability of HLE, HuH7, and HuH6 cells is significantly decreased by procaine treatment. Inhibition of S/G2/M transition, morphological changes such as vacuolation and no increase in apoptosis rate are observed in the procaine-treated HLE cells. All the genes transcriptionally suppressed by DNA hypermethylation are demethylated and reactivated with procaine treatment. Procaine has growth-inhibitory and demethylating effects on human hepatoma cells[2].
In vivo	Procaine has a growth-inhibitory and demethylating effect against xenograft in vivo[2].
Kinase Assay	Biochemical Assays: Compound potency is also assessed through incorporation of 3H-SAM into a biotinylated H3 peptide. Specifically, PRC2 containing either EZH1 (160 pM), wt EZH2 (40 pM), or Y641N mutant EZH2 (80 pM, both EZH2 prepared in-house) is pre-incubated with 3H-SAM (0.9 μM), 2 μM H3K27me3 activating peptide (H2N-RKQLATKAAR(Kme3)SAPATGGVKKP-amide) and compounds (as 10 point duplicate dose response titrations) for 120 min in a buffer consisting of 50 mM Tris (pH 8.5), 1 mM DTT, 0.07 mM Brij-35, 0.1% BSA, and 0.8% DMSO in a total volume of 12.5 μl in a black 384 well plate. Reaction is initiated with biotinylated H3 substrate peptides (H3K27me1 for wt EZH2, H3K27me2 for Y641N mutant EZH2; H2N-RKQLATKAAR(Kmen)SAPATGGVKKP-NTPEGBiot) as a 2 μM stock in 12.5 μL and allowed to react at room temperature for 5 h. Quenching is accomplished by addition of 20 μl of STOP solution (50 mM Tris (pH 8.5), 200 mM EDTA, 2 mM SAH). 35 μL of the quenched solution is transferred to Streptavidin Flashplates, incubated overnight, washed, and read in a TopCount Reader. For titrations all compound dilutions are in DMSO, final DMSO concentrations are 0.8% (v/v), and turnover is kept to less than < 5%. IC50s are calculated using non-linear least square four parameter fits (GraphPad 6.0).
Cell Research	For apoptosis analysis, the TUNEL assay is performed on HLE cells treated with no drug(control), or 1 μM of DAC(5-aza-2'-deoxycytidine), or 1 mM of PCA(procaine), or 1 mM of PCAA(procainamide) for 96 h. The proportion of TUNEL-positive cells was calculated by counting at least 500 cells randomly. All assays were carried out in triplicate. (Only for Reference)

Synonyms	Duracaine, Spinocaine, Novocaine, Vitamin H3
Molecular Weight	236.31
Formula	C13H20N2O2
CAS No.	59-46-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 47 mg/mL (198.89 mM)

Ethanol: 43 mg/mL(182 mM)

References and Literature

1. Rigon AR, et al. Gen Pharmacol. 1996, 27(4):647-50. 2. Tada M, et al. Hepatol Int. 2007, 1(3):355-64.

Related compound libraries

This product is contained In the following compound libraries：

Target-Focused Phenotypic Screening Library Immunology/Inflammation Compound Library ReFRAME Related Library Human Metabolite Library Cell Cycle Compound Library Bioactive Compound Library Anti-Alzheimer's Disease Compound Library Drug-Fragment Library Anti-Cancer Compound Library DNA Damage & Repair Compound Library

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GSK J5 LSD1-IN-5 Vafidemstat GSK2879552 2HCl (1401966-69-5(free base)) GSK-LSD1 dihydrochloride Procaine hydrochloride OG-L002 (rel)-Tranylcypromine D5 hydrochloride

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Keywords

Procaine 59-46-1 Cell Cycle/Checkpoint Chromatin/Epigenetic DNA Damage/DNA Repair Membrane transporter/Ion channel DNA/RNA Synthesis Sodium Channel Histone Demethylase targets Inhibitor Duracaine onset inhibit multiple Spinocaine Novocaine Vitamin H3 duration inhibitor

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