Home Tools

Pracinostat

Catalog No. T1890 CAS 929016-96-6

Synonyms: SB939

Pracinostat (SB939) is a novel HDAC inhibitor with improved in vivo properties compared to other HDAC inhibitors currently in Clinicalal trials, allowing oral dosing. Data demonstrate that Pracinostat is a potent and effective anti-tumor drug with potential as an oral therapy for a variety of human hematological and solid tumors.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Pracinostat, CAS 929016-96-6

Pack Size	Availability	Price/USD
1 mg	In stock	$ 43.00
2 mg	In stock	$ 62.00
5 mg	In stock	$ 98.00
10 mg	In stock	$ 178.00
25 mg	In stock	$ 297.00
50 mg	In stock	$ 449.00
100 mg	In stock	$ 663.00
500 mg	In stock	$ 1,390.00
1 mL * 10 mM (in DMSO)	In stock	Inquiry

Bulk Inquiry

Select Batch

Purity: 99.65%

Purity: 99.25%

Purity: 99.15%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Pracinostat (SB939) is a novel HDAC inhibitor with improved in vivo properties compared to other HDAC inhibitors currently in Clinicalal trials, allowing oral dosing. Data demonstrate that Pracinostat is a potent and effective anti-tumor drug with potential as an oral therapy for a variety of human hematological and solid tumors.
Targets&IC50	HDAC1:49 nM, HDAC10:40 nM, HDAC3:43 nM, HDAC5:47 nM, HDAC4:56 nM
In vitro	Administered at a dose of 50 mg/kg, SB939 selectively stimulates the growth of CT-116 xenograft tissues. It exhibits a tumor growth inhibition rate of 94% compared to SAHA. In nude mice, SB939 demonstrates superior pharmacokinetics and oral bioavailability than SAHA. Additionally, it inhibits adenoma formation in APC^min mice and enhances hematocrit levels more effectively than 5-fluorouracil. SB939 exhibits anticancer activity and high efficacy in various tumor models, including HCT-116, PC-3, A2780, MV4-11, and Ramos.
In vivo	SB939 exhibits selectivity for HDAC over non-HDAC enzymes, receptors, and ion channels that bind zinc by a factor of 100. It significantly inhibits Class II HDAC isoforms including HDAC4, HDAC5, HDAC7, HDAC9, and HDAC10, with IC50 values ranging from 40 nM to 137 nM, but does not affect HDAC6, as demonstrated by its IC50 of 1008 nM. Additionally, SB939 effectively inhibits Class IV enzyme HDAC11, with an IC50 of 93 nM, yet it lacks inhibitory activity against Class III HDACs, specifically SIRT1. Demonstrating significant antiproliferative activity against various tumor cell lines, especially leukemia cells and skin T-cell lymphoma cells, SB939's IC50 values range from 50 nM (in H9 cells) to 170 nM (in HEL92.1.7 cells).
Kinase Assay	HDAC enzyme assay: All recombinant HDAC enzymes, with the exception of SIRT1, are cloned and expressed in S*BIO. The reaction mix containing 2.5 or 5 μL of the HDAC isoenzyme, assay buffer (25 mM Tris-HCl, pH 7.5; 137 mM NaCl; 2.7 mM KCl, 1 mM MgCl2 and 1 mg/mL BSA), different concentrations of SB939, and the fluorogenic deacetylase substrate Flour de LysTM in a total reaction volume of 33 μL is incubated at room temperature for 2 hours. 16 μL of Flour de LysTM developer is added and incubated for an additional 10 minutes. The emitted light is measured at 460 nm in a microplate reader. IC50 values are generated using the XLfit software.
Cell Research	Cells are seeded in 96-well plates in the log growth phase at a predetermined optimal density, and rested for 24 hours (adherent cells) or 2 hours (suspension cells), respectively. They are exposed to different concentrations of SB939 for 96 hours. Cell proliferation assays are done using either the CyQUANT cell proliferation assay kit for adherent cells or the CellTiter96 Aqueous One solution cell proliferation kit for suspension cells.(Only for Reference)

Synonyms	SB939
Molecular Weight	358.48
Formula	C20H30N4O2
CAS No.	929016-96-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 26 mg/mL (72.5 mM)

DMSO: 67 mg/mL (186.9 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Novotny-Diermayr V, et al. Mol Cancer Ther, 2010, 9(3), 642-652.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Inhibitor Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Anti-Cancer Approved Drug Library Anti-Cancer Drug Library Anti-COVID-19 Compound Library NF-κB Signaling Compound Library Human Metabolite Library FDA-Approved & Pharmacopeia Drug Library

Related Products

Related compounds with same targets

Costunolide Diosgenin glucoside Urolithin A Liensinine Perchlorate Evocarpine Apitolisib Topoisomerase II inhibitor 14 Zibotentan

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Pracinostat 929016-96-6 Apoptosis Chromatin/Epigenetic DNA Damage/DNA Repair HDAC Inhibitor cancer SB939 inhibit SB-939 MBLAC2 SB 939 Histone deacetylases inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.