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NB-598

Catalog No. TQ0115 CAS 131060-14-5

NB-598 is an effective and competitive inhibitor of squalene epoxidase. It suppresses triglyceride biosynthesis through the farnesol pathway.

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NB-598, CAS 131060-14-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 72.00
2 mg	In stock	$ 109.00
5 mg	In stock	$ 197.00
10 mg	In stock	$ 297.00
25 mg	In stock	$ 492.00
50 mg	In stock	$ 718.00
100 mg	In stock	$ 987.00
500 mg	In stock	$ 1,980.00
1 mL * 10 mM (in DMSO)	In stock	$ 226.00

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Purity: 100%

Biological Description

Chemical Properties

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Description	NB-598 is an effective and competitive inhibitor of squalene epoxidase. It suppresses triglyceride biosynthesis through the farnesol pathway.
In vitro	NB-598 (10 μM) inhibits the synthesis of sterol and sterol ester from [14C]acetate without affecting the synthesis of other lipids such as phospholipids (PL), free fatty acids (FFA) and triacylglycerol (TG). In the absence of exogenous liposomal cholesterol, NB-598 reduces ACAT activity by 31%. NB-598 reduces ACAT activity by 22% even in the presence of a 600 pM concentration of liposomal cholesterol [1]. NB598 (10 μM) causes a 36±7% reduction in the total cholesterol level of MIN6 cells. NB598 causes a significant decrease in cholesterol by 49±2%, 46±7%, and 48±2% from PM, ER, and SG, respectively. NB598 dose-dependently inhibits insulin secretion under both basal (1 mM glucose) and glucose-stimulated (16.7 mM glucose) conditions. NB598 at concentrations up to 10 μM does not affect peak outward KV currents or the voltage dependence of activation but increases current inactivation [2].
Kinase Assay	Caco-2 cells are grown in a 58 cm2 plastic dish with medium A for 13 days. The cells are washed with medium B, and then cultured with medium B including cholesterol-micelle and each compound. The compound is dissolved in Me2SO, and the final concentration of Me2SO is 0.1%(v/v). After 18 hr of incubation, the cells are washed extensively with phosphate-buffered saline (PBS) to remove the compound. Microsomes are prepared as described above. The reaction mixture (0.2 mL) consisted of 0.1 mg microsomes, 0.25% BSA and 40 PM [14C]oleoyl CoA in buffer A. To avoid the effects of endogenous cholesterol, liposome (2 mol of cholesterol: 1 mol of phosphatidylcholine) [15] is added to the reaction mixture. The microsomes are preincubated for 1 hr with or without exogenous cholesterol, and ACAT activity is determined as described above [1].

Molecular Weight	449.67
Formula	C27H31NOS2
CAS No.	131060-14-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 10 mg/mL (22.24 mM)

References and Literature

1. Horie M, et al. Effects of NB-598, a potent squalene epoxidase inhibitor, on the apical membrane uptake of cholesterol and basolateral membrane secretion of lipids in Caco-2 cells. Biochem Pharmacol. 1993 Jul 20;46(2):297-305. 2. Xia F, et al. Inhibition of cholesterol biosynthesis impairs insulin secretion and voltage-gated calcium channel function in pancreatic beta-cells. Endocrinology. 2008 Oct;149(10):5136-45.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Infection Compound Library Bioactive Compounds Library Max Bioactive Compound Library Anti-Cardiovascular Disease Compound Library Preclinical Compound Library Covalent Inhibitor Library Antibiotics Library ReFRAME Related Library NO PAINS Compound Library

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Keywords

NB-598 131060-14-5 Microbiology/Virology Antifungal inhibit Inhibitor NB598 NB 598 inhibitor

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