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Hydroxocobalamin monohydrochloride

Catalog No. T13731 CAS 59461-30-2

Synonyms: Vitamin B12a monohydrochloride

Hydroxocobalamin monohydrochloride is an injectable naturally occurring form of vitamin B12. It is used as a dietary supplement in the treatment of vitamin B12 deficiency including pernicious anemia.

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Hydroxocobalamin monohydrochloride Chemical Structure

Hydroxocobalamin monohydrochloride, CAS 59461-30-2

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	Hydroxocobalamin monohydrochloride is an injectable naturally occurring form of vitamin B12. It is used as a dietary supplement in the treatment of vitamin B12 deficiency including pernicious anemia.
In vitro	Hydroxocobalamin monohydrochloride ?is an injectable naturally occurring form of vitamin B12 with a favorable adverse effect profile.Hydroxocobalamin monohydrochloride ?used as a dietary supplement in the treatment of vitamin B12 deficiency including pernicious anemia.
In vivo	Administering hydroxocobalamin after NaSH (sodium hydrosulfide) significantly improves survival rates in mice; over 60% survive with hydroxocobalamin treatment compared to less than 15% without it. This compound, at concentrations of 50–100 μM, along with cobalt at the same concentrations, also mitigates NaSH-induced hepatocyte cytotoxicity. Even if hydroxocobalamin is introduced 60 minutes post-NaSH administration, it retains protective efficacy. Additionally, pretreatment or post-treatment with hydroxocobalamin lessens the severity of LPS (lipopolysaccharide)-induced hypotension, modulates plasma RNI (reactive nitrogen intermediates) levels, and boosts LPS-induced RNI urinary excretion. In a specific study, hydroxocobalamin administration 30 minutes before LPS exposure reduced the 24-hour mortality rate from 80% to 50%, and the 36- and 96-hour mortality rates from 100% to 60% in Swiss-Webster mice.

Synonyms	Vitamin B12a monohydrochloride
Molecular Weight	1382.82
Formula	C62H90ClCoN13O15P
CAS No.	59461-30-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 100 mg/mL (72.32 mM), Sonication is recommended.

H2O: 25 mg/mL (18.08 mM), Sonication is recommended.

References and Literature

1. Cheungpasitporn W, et al. High-dose hydroxocobalamin for vasoplegic syndrome causing false blood leak alarm. Clin Kidney J. 2017 Jun;10(3):357-362. 2. Wang H, et al. Oral vitamin B12 versus intramuscular vitamin B12 for vitamin B12 deficiency. Cochrane Database Syst Rev. 2018 Mar 15;3:CD004655. 3. Greenberg SS, et al. Hydroxocobalamin (vitamin B12a) prevents and reverses endotoxin-induced hypotension and mortality in rodents: role of nitric oxide. J Pharmacol Exp Ther. 1995 Apr;273(1):257-65. 4. Truong DH, et al. Prevention of hydrogen sulfide (H2S)-induced mouse lethality and cytotoxicity by hydroxocobalamin (vitamin B(12a)). Toxicology. 2007 Dec 5;242(1-3):16-22.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Hydroxocobalamin monohydrochloride 59461-30-2 Others Vitamin B12a Vitamin B12a monohydrochloride Vitamin B12a Monohydrochloride Hydroxocobalamin Hydroxocobalamin Monohydrochloride inhibitor inhibit

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