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Ferrostatin-1

Catalog No. T6500   CAS 347174-05-4
Synonyms: Ferrostatin 1, Ferrostatin-1 (Fer-1)

Ferrostatin-1 (Fer-1) is a potent ferroptosis inhibitor (EC50: 60 nM).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Ferrostatin-1 Chemical Structure
Ferrostatin-1, CAS 347174-05-4
Pack Size Availability Price/USD Quantity
5 mg In stock $ 59.00
10 mg In stock $ 89.00
25 mg In stock $ 189.00
50 mg In stock $ 329.00
200 mg In stock $ 558.00
500 mg In stock $ 897.00
1 mL * 10 mM (in DMSO) In stock $ 65.00
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Purity: 99.68%
Purity: 99.51%
Purity: 99.07%
Purity: 96.1%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Ferrostatin-1 (Fer-1) is a potent ferroptosis inhibitor (EC50: 60 nM).
Targets&IC50 ferroptosis:60 nM(EC50, HT-1080 cells)
In vitro Ferrostatin-1 (Fer-1) is the most potent inhibitor of erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1 did prevent erastin-induced accumulation of cytosolic and lipid ROS [1]. Ferrostatin-1 inhibited cell death in cellular models of Huntington's disease (HD), periventricular leukomalacia (PVL), and kidney dysfunction; Fer-1 inhibited lipid peroxidation, but not mitochondrial reactive oxygen species formation or lysosomal membrane permeability [2]. Fer-1 is not toxic toward the SH-SY5Y cells at concentrations up to 12.5 μM. Fer-1 compound quenched the commercially available stable radical DPPH, in non-cellular assay at 82 %. Fer-1 inhibited the ROS/RNS generated under rotenone insult in SH-SY5Y cells [3].
Cell Research Cell viability was typically assessed in 384-well format by Alamar Blue fluorescence (ex/em 530/590) measured on a Victor3 plate reader. In some experiments, Trypan Blue dye exclusion counting was performed using an automated cell counter. Cell viability under test conditions is reported as a percentage relative to the negative control treatment [1].
Synonyms Ferrostatin 1, Ferrostatin-1 (Fer-1)
Molecular Weight 262.35
Formula C15H22N2O2
CAS No. 347174-05-4

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 26.2 mg/mL (100 mM)

DMSO: 200 mg/mL (762.3 mM)

TargetMolReferences and Literature

1. Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72. 2. Skouta R, et al. Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models. J Am Chem Soc. 2014 Mar 26;136(12):4551-6. 3. Kabiraj P, et al. The neuroprotective role of ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic neuroblastoma cells. Protein J. 2015 Oct;34(5):349-58. 4. Su G, Yang W, Wang S, et al. SIRT1-autophagy axis inhibits excess iron-induced ferroptosis of foam cells and subsequently increases IL-1Β and IL-18[J]. Biochemical and Biophysical Research Communications. 2021, 561: 33-39. 5. Sun Y, He L, Wang T, et al. Activation of p62-Keap1-Nrf2 Pathway Protects 6-Hydroxydopamine-Induced Ferroptosis in Dopaminergic Cells[J]. Molecular Neurobiology. 2020, 57(11): 4628-4641. 6. Chen X, Gao C, Cheng Z, et al. Ruxolitinib exerts neuroprotection via repressing ferroptosis in a mouse model of traumatic brain injury[J]. Experimental Neurology. 2021: 113762. 7. Yin W, Luan X, Li Z, et al. Structural basis for inhibition of the SARS-CoV-2 RNA polymerase by suramin[J]. Nature Structural & Molecular Biology. 2021: 1-7. 8. Wang S, Li F, Qiao R, et al. Arginine-Rich Manganese Silicate Nanobubbles as a Ferroptosis-Inducing Agent for Tumor-Targeted Theranostics[J]. ACS nano. 2018 Dec 26;12(12):12380-12392.

TargetMolCitations

1. Hu G, Cui Z, Chen X, et al.Suppressing Mesenchymal Stromal Cell Ferroptosis Via Targeting a Metabolism‐Epigenetics Axis Corrects their Poor Retention and Insufficient Healing Benefits in the Injured Liver Milieu.Advanced Science.2023: 2206439. 2. Bi G, Liang J, Shan G, et al.Retinol saturase mediates retinoid metabolism to impair a ferroptosis defense system in cancer cells.Cancer Research.2023: CAN-22-3977. 3. Li Y, Yang W, Zheng Y, et al.Targeting fatty acid synthase modulates sensitivity of hepatocellular carcinoma to sorafenib via ferroptosis.Journal of Experimental & Clinical Cancer Research.2023, 42(1): 1-19. 4. Xiang P, Chen Q, Chen L, et al.Metabolite Neu5Ac triggers SLC3A2 degradation promoting vascular endothelial ferroptosis and aggravates atherosclerosis progression in ApoE-/-mice.Theranostics.2023, 13(14): 4993. 5. Zeng S T, Shao W, Yu Z Y, et al.Construction of a TICT-AIE-Integrated Unimolecular Platform for Imaging Lipid Droplet–Mitochondrion Interactions in Live Cells and In Vivo.ACS Sensors.2022 6. Wang X, Ji Y, Qi J, et al.Mitochondrial carrier 1 (MTCH1) governs ferroptosis by triggering the FoxO1-GPX4 axis-mediated retrograde signaling in cervical cancer cells.Cell Death & Disease.2023, 14(8): 1-13. 7. Tan Q, Zhang X, Li S, et al.DMT1 differentially regulates mitochondrial complex activities to reduce glutathione loss and mitigate ferroptosis.Free Radical Biology and Medicine.2023 8. Chen T, Leng J, Tan J, et al.Discovery of Novel Potent Covalent Glutathione Peroxidase 4 Inhibitors as Highly Selective Ferroptosis Inducers for the Treatment of Triple-Negative Breast Cancer.Journal of Medicinal Chemistry.2023 9. Wu Z, Lin C, Zhang F, et al.TIGD1 Function as a Potential Cuproptosis Regulator Following a Novel Cuproptosis-Related Gene Risk Signature in Colorectal Cancer.Cancers.2023, 15(8): 2286. 10. Zhao G, Liang J, Shan G, et al.KLF11 regulates lung adenocarcinoma ferroptosis and chemosensitivity by suppressing GPX4.Communications Biology.2023, 6(1): 570.
11. Wu Y T, Zhong L S, Huang C, et al.β-Caryophyllene Acts as a Ferroptosis Inhibitor to Ameliorate Experimental Colitis.International Journal of Molecular Sciences.2022, 23(24): 16055. 12. Wang Y, Li B, Liu G, et al.Corilagin attenuates intestinal ischemia/reperfusion injury in mice by inhibiting ferritinophagy-mediated ferroptosis through disrupting NCOA4-ferritin interaction.Life Sciences.2023: 122176. 13. Liu Q, Song T, Chen B, et al.Ferroptosis of brain microvascular endothelial cells contributes to hypoxia‐induced blood–brain barrier injury.The FASEB Journal.2023, 37(5): e22874. 14. Liu J, Pan Z, Tong B, et al.Artesunate protects against ocular fibrosis by suppressing fibroblast activation and inducing mitochondria‐dependent ferroptosis.The FASEB Journal.2023, 37(6): e22954. 15. Ling M, Ye L, Zeng Q, et al.Ferrostatin-1 alleviates ventilator-induced lung injury by inhibiting ferroptosis.International Immunopharmacology.2023, 120: 110356. 16. Jiang X, Teng X, Shi H, et al.Discovery and optimization of olanzapine derivatives as new ferroptosis inhibitors.Bioorganic Chemistry.2023: 106393. 17. Li J, Lu Q, Peng M, et al.Water extract from Herpetospermum pedunculosum attenuates oxidative stress and ferroptosis induced by acetaminophen via regulating Nrf2 and NF-κB pathways.Journal of Ethnopharmacology.2023, 305: 116069. 18. Zhao C, Wang C, Liu M, et al.Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis.Scientific Reports.2023, 13(1): 6833. 19. Shi H, Jiang X, Cao L, et al.Chemical constituents of Ajuga forrestii and their anti-ferroptosis activity.Fitoterapia.2023: 105461. 20. Wang Z Q, Li Y Q, Wang D Y, et al.Natural product piperlongumine inhibits proliferation of oral squamous carcinoma cells by inducing ferroptosis and inhibiting intracellular antioxidant capacity.Transl Cancer Res.2022 21. Wang S, Li F, Qiao R, et al. Arginine-Rich Manganese Silicate Nanobubbles as a Ferroptosis-Inducing Agent for Tumor-Targeted Theranostics. ACS nano. 2018 Dec 26;12(12):12380-12392. 22. Ouyang S, Li H, Lou L, et al. Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer. Redox Biology. 2022: 102317 23. Li W, Luo L X, Zhou Q Q, et al. Phospholipid peroxidation inhibits autophagy via stimulating the delipidation of oxidized LC3-PE. Redox Biology. 2022: 102421. 24. Yin W, Luan X, Li Z, et al. Structural basis for inhibition of the SARS-CoV-2 RNA polymerase by suramin. Nature Structural & Molecular Biology. 2021: 1-7. 25. Mao W, Cai Y, Chen D, et al. Statin shapes inflamed tumor microenvironment and enhances immune checkpoint blockade in non-small cell lung cancer. JCI insight. 2022 26. Bi G, Liang J, Zhao M, et al. MiR-6077 promotes cisplatin/pemetrexed resistance in lung adenocarcinoma by targeting CDKN1A/cell cycle arrest and KEAP1/ferroptosis pathways. Molecular Therapy-Nucleic Acids. 2022 27. Yang Y, Ma Y, Li Q, et al. STAT6 inhibits ferroptosis and alleviates acute lung injury via regulating P53/SLC7A11 pathway. Cell Death & Disease. 2022, 13(6): 1-14 28. Wang F, Xie M, Chen P, et al. Homoharringtonine combined with cladribine and aclarubicin (HCA) in acute myeloid leukemia: A new regimen of conventional drugs and its mechanism. Oxidative Medicine and Cellular Longevity. 2022 29. Fang Y, Tan Q, Zhou H, et al. Discovery and optimization of 2-(trifluoromethyl) benzimidazole derivatives as novel ferroptosis inducers in vitro and in vivo. European Journal of Medicinal Chemistry. 2022: 114905. 30. Luo Y, Gao X, Zou L, et al. Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells. Oxidative Medicine and Cellular Longevity. 2021 31. Jiang H, Wang C, Zhang A, et al. ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis. Biomedicine & Pharmacotherapy. 2022, 153: 113280 32. Yu S, Li Z, Zhang Q, et al. GPX4 degradation via chaperone-mediated autophagy contributes to antimony-triggered neuronal ferroptosis. Ecotoxicology and Environmental Safety. 2022, 234: 113413. 33. Zhou Y, Wu H, Wang F, et al. GPX7 Is Targeted by miR-29b and GPX7 Knockdown Enhances Ferroptosis Induced by Erastin in Glioma. Frontiers in oncology. 2021, 11: 802124-802124. 34. Li J, Chen S, Huang J, et al. New Target in an Old Enemy: Herbicide (R)-Dichlorprop Induces Ferroptosis-like Death in Plants. Journal of Agricultural and Food Chemistry. 2021 35. Zhao Y, Lu J, Mao A, et al. Autophagy Inhibition Plays a Protective Role in Ferroptosis Induced by Alcohol via the p62–Keap1–Nrf2 Pathway. Journal of Agricultural and Food Chemistry. 2021 36. Tan Q, Fang Y, Peng X, et al. A new ferroptosis inhibitor, isolated from Ajuga nipponensis, protects neuronal cells via activating NRF2-antioxidant response elements (AREs) pathway. Bioorganic Chemistry. 2021: 105177. 37. Ning X, Qi H, Yuan Y, et al. Identification of a new small molecule that initiates ferroptosis in cancer cells by inhibiting the system Xc− to deplete GSH. European Journal of Pharmacology. 2022: 175304. 38. Kong R, Wang N, Han W, et al. IFNγ‐mediated repression of system xc− drives vulnerability to induced ferroptosis in hepatocellular carcinoma cells. Journal of Leukocyte Biology. 2021, 110(2): 301-314. 39. Yang S, Pei T, Wang L, et al. Salidroside Alleviates Renal Fibrosis in SAMP8 Mice by Inhibiting Ferroptosis. Molecules. 2022, 27(22): 8039. 40. Peng X, Tan Q, Wu L, et al. Ferroptosis Inhibitory Aromatic Abietane Diterpenoids from Ajuga decumbens and Structural Revision of Two 3, 4-Epoxy Group-Containing Abietanes. Journal of Natural Products. 2022 41. Chen X, Gao C, Cheng Z, et al. Ruxolitinib exerts neuroprotection via repressing ferroptosis in a mouse model of traumatic brain injury. Experimental Neurology. 2021: 113762. 42. Yang S, Pei T, Xiong Q, et al. Salidroside attenuates neuronal ferroptosis by activating the Nrf2/HO1 signaling pathway in Aβ1-42-induced Alzheimer’s disease mice and glutamate-injured HT22 cells. Chinese Medicine. 2022, 17(1): 1-18 43. Sun Y, He L, Wang T, et al. Activation of p62-Keap1-Nrf2 Pathway Protects 6-Hydroxydopamine-Induced Ferroptosis in Dopaminergic Cells. Molecular Neurobiology. 2020, 57(11): 4628-4641. 44. Tang X, Li X, Zhang D, et al. Astragaloside-IV alleviates high glucose-induced ferroptosis in retinal pigment epithelial cells by disrupting the expression of miR-138-5p/Sirt1/Nrf2. Bioengineered. 2022, 13(4): 8240-8254. 45. Su G, Yang W, Wang S, et al. SIRT1-autophagy axis inhibits excess iron-induced ferroptosis of foam cells and subsequently increases IL-1Β and IL-18. Biochemical and Biophysical Research Communications. 2021, 561: 33-39. 46. Peng, Xing, et al. Discovery of phloroglucinols from Hypericum japonicum as ferroptosis inhibitors. Fitoterapia. (2021): 104984. 47. Shan G, Bi G, Zhao G, et al.Inhibition of PKA/CREB1 pathway confers sensitivity to ferroptosis in non-small cell lung cancer.Respiratory Research.2023, 24(1): 1-15. 48. Wang J, Yan J T, Zeng S T, et al.Revealing Mitochondrion–Lysosome Dynamic Interactions and pH Variations in Live Cells with a pH-Sensitive Fluorescent Probe.Analytical Chemistry.2023 49. Wang Y, He X, Xue M, et al.Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy.Free Radical Research.2023 (just-accepted): 1-36. 50. Liu L, Luo C, Zheng D, et al.TRPML1 contributes to antimony-induced nephrotoxicity by initiating ferroptosis via chaperone-mediated autophagy.Food and Chemical Toxicology.2023: 114378.
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Related compound libraries

This product is contained In the following compound libraries:
Ferroptosis Compound Library Cell Cycle Compound Library Bioactive Compound Library Anti-Infection Compound Library Antioxidant Compound Library Anti-Metabolism Disease Compound Library Anti-Fungal Compound Library Apoptosis Compound Library Inhibitor Library NO PAINS Compound Library

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Keywords

Ferrostatin-1 347174-05-4 Apoptosis Microbiology/Virology Ferroptosis Antifungal ROS inhibit Fer1 cell neurotoxicity cytosolic Ferrostatin1 Fungal antifungal antioxidant Ferrostatin 1 Fer 1 Ferrostatin-1 (Fer-1) death Inhibitor Fer-1 inhibitor

 

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