Home Tools

Brassinolide

Catalog No. T3151 CAS 72962-43-7

Synonyms: Brassin lactone, 24-Epibrassinolide

Brassinolide (Brassin lactone) is a plant hormone isolated from rapeseed (Brassica napus) promoting stem elongation and cell division.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Brassinolide, CAS 72962-43-7

Pack Size	Availability	Price/USD
5 mg	In stock	$ 39.00
10 mg	In stock	$ 64.00
25 mg	In stock	$ 128.00
50 mg	In stock	$ 213.00
100 mg	In stock	$ 347.00
1 mL * 10 mM (in DMSO)	In stock	$ 41.00

Bulk Inquiry

Select Batch

Purity: 99.62%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Brassinolide (Brassin lactone) is a plant hormone isolated from rapeseed (Brassica napus) promoting stem elongation and cell division.
In vitro	Brassinolide, a plant sterol isolated from rape pollen (Brassica napus L.), demonstrates both growth-modulating properties and potential anti-cancer activities. In PC-3 cells, Brassinolide induces cytotoxicity in a time and concentration-dependent manner, primarily through apoptosis. This is evidenced by increased Caspase-3 activity, and a decrease in the anti-apoptotic protein Bcl-2, as confirmed by various microscopy techniques and flow-cytometric analysis. These findings position Brassinolide as a promising candidate for prostate cancer treatment[1]. Furthermore, Brassinolide shows efficacy in reversing multidrug resistance (MDR) in the human T lymphoblastoid cell line CCRF-VCR, which develops resistance through exposure to vincristine (VCR). Treatment with Brassinolide at concentrations between 0.001-10 μg/mL partially reverses this resistance and enhances the intracellular accumulation of rhodamine 123, indicating a reduction in drug efflux mediated by P-glycoprotein. Despite showing no change in topoisomerase II activity, Brassinolide treatment normalizes the elevated levels of p53 protein in resistant cells to those seen in sensitive cells. This suggests that modulating p53 expression could be a mechanism by which Brassinolide reverses MDR[2].
Cell Research	Brassinolide is dissolved in DMSO and stored, and then diluted with appropriate medium before use[2]. MTT method is used to detect the resistant factor of resistant cell line and the reversing fold after addition of Brassinolide. The intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein is detected by flow cytometry, the catalytic activity of topoisomerase II is assessed by Sulliven method to find the effect of Brassinolide on resistance. The protein expression of p53 is measured using Western blotting in the sensitive cells and resistant cells to explore the effect of Brassinolide[2].
Source	Natural Products > Brassicaceae > Brassica

Synonyms	Brassin lactone, 24-Epibrassinolide
Molecular Weight	480.68
Formula	C28H48O6
CAS No.	72962-43-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 60 mg/mL (124.82 mM)

References and Literature

1. Wu YD, et al. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie. 2007 May;62(5):392-5. 2. Xian LJ, et al. Reversing effect of brassinolide on multidrug resistance of-CCRF-VCR12000 cells and a preliminary investigation on its mechanisms. Yao Xue Xue Bao. 2005 Feb;40(2):117-21.

Related compound libraries

This product is contained In the following compound libraries：

Tobacco Monomer Library Traditional Chinese Medicine Monomer Library Apoptosis Compound Library Anti-Tumor Natural Product Library Natural Product Library for HTS Natural Product Library Human Endogenous Metabolite Compound Library Plus Selected Plant-Sourced Compound Library NO PAINS Compound Library Bioactive Compound Library

Related Products

Related compounds with same targets

3-Dehydrotrametenolic acid Antineoplaston A10 D609 AZD5582 Targapremir-210 Dihydrorotenone (-)-Zeylenol β-Ionone

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Brassinolide 72962-43-7 Apoptosis Others Brassin lactone 24-Epibrassinolide Inhibitor inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.