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Agerafenib

Agerafenib
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Purity:99.23%
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Agerafenib

Catalog No. T2070Cas No. 1188910-76-0
Agerafenib (CEP32496) is a highly potent inhibitor of BRAF.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
1 mg$38In Stock
2 mg$54In Stock
5 mg$89In Stock
10 mg$133In Stock
25 mg$243In Stock
50 mg$397In Stock
100 mg$593In Stock
1 mL x 10 mM (in DMSO)$97In Stock
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Product Introduction

Bioactivity
Description
Agerafenib (CEP32496) is a highly potent inhibitor of BRAF.
Kinase Assay
Binding assay: Kinases are produced displayed on T7 phage or by expression in HEK-293 cells and tagged with DNA. Binding reactions are performed at room temperature for 1 hour, and the fraction of kinase not bound to test compound is determined by capture with an immobilized affinity ligand and quantitation by quantitative PCR. Each kinase is tested individually against CEP-32496. Kd values are determined using eleven serial 3-fold dilutions and presented as mean values from experiments performed in duplicate. Variability between individual values is less than 2-fold.
Cell Research
Cells are seeded at 104 cells per well in DMEM with 10% fetal calf serum and allowed to attach. The cells are washed with PBS and switched to DMEM with 0.5% of serum and incubated overnight. CEP-32496 is then added at various concentrations with a final DMSO concentration of 0.5% and incubated for 72 h. At the end of incubation, a Cell Titer Blue is added per instructions, and incubation is continued for 3 hours. Remaining viable cells are quantified by measuring the strength of the fluorescence signal using SoftMax Pro (excitation at 560 nm and emission at 590 nm). IC50 values are derived using a 9-point curve fitted with Igor Pro and are presented as mean values from experiments performed in duplicate. Variability between individual values is less than 2-fold.(Only for Reference)
AliasRXDX-105, CEP 32496, CEP-32496, CEP32496
Chemical Properties
Molecular Weight517.46
FormulaC24H22F3N5O5
Cas No.1188910-76-0
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 45 mg/mL (86.96 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
100 mM0.0193 mL0.0966 mL0.1933 mL0.9663 mL

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Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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