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API-1

Catalog No. T8969 CAS 36707-00-3

Synonyms: NSC177223

API-1 (NSC-177223) is a potent inhibitor of Akt. It induces GSK3-dependent, β-TrCP- and FBXW7-mediated Mcl-1 degradation, resulting in induction of apoptosis .

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API-1, CAS 36707-00-3

Pack Size	Availability	Price/USD
1 mg	In stock	$ 130.00
5 mg	In stock	$ 265.00
10 mg	In stock	$ 437.00
25 mg	In stock	$ 723.00
50 mg	In stock	$ 987.00
100 mg	In stock	$ 1,380.00
1 mL * 10 mM (in DMSO)	In stock	$ 297.00

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Purity: 99.07%

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Description

API-1 (NSC-177223) is a potent inhibitor of Akt. It induces GSK3-dependent, β-TrCP- and FBXW7-mediated Mcl-1 degradation, resulting in induction of apoptosis .

In vitro

API-1 rapidly and potently reduced the levels of Mcl-1 primarily in API-1-senstive lung cancer cell lines.?Ectopic expression of Mcl-1 protected cells from induction of apoptosis by API-1.?API-1 treatment decreased the half-life of Mcl-1, whereas inhibition of the proteasome with MG132 rescued Mcl-1 reduction induced by API-1.?API-1 decreased Mcl-1 levels accompanied with a rapid increase in Mcl-1 phosphorylation (S159/T163).?Moreover, inhibition of GSK3 inhibited Mcl-1 phosphorylation and reduction induced by API-1 and antagonized the effect of API-1 on induction of apoptosis.

Synonyms	NSC177223
Molecular Weight	337.29
Formula	C13H15N5O6
CAS No.	36707-00-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 22.5 mg/mL (66.71 mM)

References and Literature

1. Ren H , Koo J , Guan B , et al. The E3 ubiquitin ligases β-TrCP and FBXW7 cooperatively mediates GSK3-dependent Mcl-1 degradation induced by the Akt inhibitor API-1, resulting in apoptosis[J]. Molecular Cancer, 2013, 12(1):146-146.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Kinase Inhibitor Library Oxidation-Reduction Compound Library Glutamine Metabolism Compound Library Anti-Colorectal Cancer Compound Library Cytoskeletal Signaling Pathway Compound Library PI3K-AKT-mTOR Compound Library Bioactive Compounds Library Max Antidepressant Compound Library Anti-Prostate Cancer Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

API-1 36707-00-3 Cytoskeletal Signaling PI3K/Akt/mTOR signaling Akt API 1 TRAIL PKB Inhibitor NSC-177223 API1 inhibit Akt/PKB inhibitor NSC177223 Protein kinase B NSC 177223 Apoptosis inhibitor

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