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Catalog No. T2352   CAS 20324-87-2

AMI-1 is an effective and selective Histone Methyltransferase (HMT) inhibitor (IC50: 3.0/8.8 μM, for yeast Hmt1p, and human PRMT1).

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AMI-1, CAS 20324-87-2
Pack Size Availability Price/USD Quantity
5 mg In stock $ 43.00
10 mg In stock $ 64.00
25 mg In stock $ 128.00
50 mg In stock $ 189.00
100 mg In stock $ 297.00
500 mg In stock $ 718.00
1 mL * 10 mM (in DMSO) In stock $ 52.00
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Purity: 99.9%
Purity: 98%
Purity: 97.53%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description AMI-1 is an effective and selective Histone Methyltransferase (HMT) inhibitor (IC50: 3.0/8.8 μM, for yeast Hmt1p, and human PRMT1).
Targets&IC50 yeast Hmt1p:3.0 μM, hμMan PRMT1:8.8 μM
In vitro In HeLa cells, AMI-1 inhibits methylation levels of GFP-Npl3 fusion and endogenous PRMT1-like activity. AMI-1 also inhibits nuclear receptor-mediated transactivation of a luciferase reporter in MCF7 cells. [1] In addition, AMI-1 inhibits HIV-1 RT polymerase activity with IC50 of 5 μM and inhibits DNA binding to HIV-1 RT with Kd of 2 μM. [2] In INS-1 cells, AMI-1 improves INS-1 cell function and mediates translocations of FOXO1 and PDX-1 intracellularly by regulating FOXO1 phosphorylation and methylation. [4]
In vivo In chronic AIPI rats, AMI-1 (5 μg/rat) ameliorates COX2 expression and asthmatic indexes, and decreases the airway and alveoli lesions, mucus secretion, and collagen deposition in lungs. [3]
Kinase Assay INS-1 832/13 cells are suspended in RPMI medium containing 11 mM glucose and the supplements described above. These cells are seeded at a density of 2×104?cells/well in a 96-well black plate coated with poly-D-lysine, and 1% BSA and 0.1% DMSO alone (control), palmitic acid (62.5, 125, 250, 500, and 1000 μM), oleic acid (62.5, 125, 250, 500, and 1000 μM), or TAK-875 (6.25, 12.5, 25, 50, and 100 μM) is added to the plate with 1% BSA and 0.1% DMSO, followed by culture for 72 h. After the culture, caspase 3/7 activity is measured with the Apo-one homogeneous caspase 3/7 assay according to the manufacturer's instructions. Fluorescence intensity is measured at an excitation of 485 nm and an emission at 535 nm.
Molecular Weight 548.45
Formula C21H14N2Na2O9S2
CAS No. 20324-87-2


Powder: -20°C for 3 years | In solvent: -80°C for 2 years

Solubility Information

Ethanol: <1 mg/mL

H2O: 10 mg/mL (18.23 mM)

DMSO: 93 mg/mL (159.1 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Cheng D, et al. J Biol Chem. 2004, 279(23), 23892-23899. 2. Skillman AG, et al. Bioorg Chem. 2002, 30(6), 443-458. 3. Sun Q, et al. J Immunol. 2015, 195(1), 298-306. 4. Lv L, et al. Endocrine. 2015,49(3), 669-682.


1. Li X, Wang Y, Zhang Y, et al. Overexpression of MCAM induced by SMYD2-H3K36me2 in breast cancer stem cell properties. Breast Cancer. 2022: 1-15 2. Feng G, Chen C, Luo Y.PRMT1 accelerates cell proliferation, migration, and tumor growth by upregulating ZEB1/H4R3me2as in thyroid carcinoma.Oncology Reports.2023, 50(6): 1-11.

Related compound libraries

This product is contained In the following compound libraries:
Stem Cell Differentiation Compound Library Histone Modification Compound Library Chromatin Modification Compound Library Reprogramming Compound Library Covalent Inhibitor Library Anti-Aging Compound Library Target-Focused Phenotypic Screening Library Bioactive Compounds Library Max Bioactive Compound Library NO PAINS Compound Library

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AMI-1 20324-87-2 Chromatin/Epigenetic Histone Methyltransferase protein arginine N-methyltransferases AMI 1 Hmt1p Inhibitor inhibit PRMT1 yeast-Hmt1p AMI1 PRMTs inhibitor