Powder: -20°C for 3 years | In solvent: -80°C for 2 years
AMI-1 is an effective and selective Histone Methyltransferase (HMT) inhibitor (IC50: 3.0/8.8 μM, for yeast Hmt1p, and human PRMT1).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 43.00 | |
10 mg | In stock | $ 64.00 | |
25 mg | In stock | $ 128.00 | |
50 mg | In stock | $ 189.00 | |
100 mg | In stock | $ 297.00 | |
500 mg | In stock | $ 718.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 52.00 |
Description | AMI-1 is an effective and selective Histone Methyltransferase (HMT) inhibitor (IC50: 3.0/8.8 μM, for yeast Hmt1p, and human PRMT1). |
Targets&IC50 | yeast Hmt1p:3.0 μM, hμMan PRMT1:8.8 μM |
In vitro | In HeLa cells, AMI-1 inhibits methylation levels of GFP-Npl3 fusion and endogenous PRMT1-like activity. AMI-1 also inhibits nuclear receptor-mediated transactivation of a luciferase reporter in MCF7 cells. [1] In addition, AMI-1 inhibits HIV-1 RT polymerase activity with IC50 of 5 μM and inhibits DNA binding to HIV-1 RT with Kd of 2 μM. [2] In INS-1 cells, AMI-1 improves INS-1 cell function and mediates translocations of FOXO1 and PDX-1 intracellularly by regulating FOXO1 phosphorylation and methylation. [4] |
In vivo | In chronic AIPI rats, AMI-1 (5 μg/rat) ameliorates COX2 expression and asthmatic indexes, and decreases the airway and alveoli lesions, mucus secretion, and collagen deposition in lungs. [3] |
Kinase Assay | INS-1 832/13 cells are suspended in RPMI medium containing 11 mM glucose and the supplements described above. These cells are seeded at a density of 2×104?cells/well in a 96-well black plate coated with poly-D-lysine, and 1% BSA and 0.1% DMSO alone (control), palmitic acid (62.5, 125, 250, 500, and 1000 μM), oleic acid (62.5, 125, 250, 500, and 1000 μM), or TAK-875 (6.25, 12.5, 25, 50, and 100 μM) is added to the plate with 1% BSA and 0.1% DMSO, followed by culture for 72 h. After the culture, caspase 3/7 activity is measured with the Apo-one homogeneous caspase 3/7 assay according to the manufacturer's instructions. Fluorescence intensity is measured at an excitation of 485 nm and an emission at 535 nm. |
Molecular Weight | 548.45 |
Formula | C21H14N2Na2O9S2 |
CAS No. | 20324-87-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Ethanol: <1 mg/mL
H2O: 10 mg/mL (18.23 mM)
DMSO: 93 mg/mL (159.1 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
AMI-1 20324-87-2 Chromatin/Epigenetic Histone Methyltransferase protein arginine N-methyltransferases AMI 1 Hmt1p Inhibitor inhibit PRMT1 yeast-Hmt1p AMI1 PRMTs inhibitor