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AMI-1 free acid

Name: AMI-1 free acid
Brand: TargetMol
SKU: T22239
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T22239Cas No. 134-47-4

AMI-1 free acid is a potent, cell-permeable, and reversible inhibitor of protein arginine N-methyltransferases (PRMTs), with inhibitory concentration 50 (IC50) values of 8.8 μM for human PRMT1 and 3.0 μM for yeast-Hmt1p. It achieves its PRMTs inhibitory activity by obstructing peptide-substrate binding [1].

AMI-1 free acid

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Purity: 97.8%

Catalog No. T22239Cas No. 134-47-4

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$40	-	In Stock
50 mg	$56	-	In Stock
100 mg	$81	-	In Stock
500 mg	$198	-	In Stock
1 g	$293	-	In Stock
1 mL x 10 mM (in DMSO)	$29	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.8%

Appearance:Solid

Color:Yellow

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	AMI-1 free acid is a potent, cell-permeable, and reversible inhibitor of protein arginine N-methyltransferases (PRMTs), with inhibitory concentration 50 (IC50) values of 8.8 μM for human PRMT1 and 3.0 μM for yeast-Hmt1p. It achieves its PRMTs inhibitory activity by obstructing peptide-substrate binding [1].
In vitro	In S180 cells and U2OS cells, AMI-1, free acid (0.6 mM, 1.2 mM, 2.4 mM; 48 hours, 72 hours, 96 hours) inhibits cell viability[3].
In vivo	AMI-1, free acid (0.5 mg; administered daily via subcutaneous injection for 7 days) reduces tumor weight in male Kunming mice with S180 cell xenografts[3].

Chemical Properties

Molecular Weight	504.49
Formula	C₂₁H₁₆N₂O₉S₂
Cas No.	134-47-4
Smiles	O=C(NC=1C=CC2=C(O)C=C(C=C2C1)S(=O)(=O)O)NC=3C=CC4=C(O)C=C(C=C4C3)S(=O)(=O)O
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (118.93 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.96 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9822 mL	9.9110 mL	19.8220 mL	99.1100 mL
5 mM	0.3964 mL	1.9822 mL	3.9644 mL	19.8220 mL
10 mM	0.1982 mL	0.9911 mL	1.9822 mL	9.9110 mL
20 mM	0.0991 mL	0.4955 mL	0.9911 mL	4.9555 mL
50 mM	0.0396 mL	0.1982 mL	0.3964 mL	1.9822 mL
100 mM	0.0198 mL	0.0991 mL	0.1982 mL	0.9911 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc