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AMI-1 free acid

Name: AMI-1 free acid
Brand: TargetMol
SKU: T22239
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T22239Cas No. 134-47-4

AMI-1 free acid is a potent, cell-permeable, and reversible inhibitor of protein arginine N-methyltransferases (PRMTs), with inhibitory concentration 50 (IC50) values of 8.8 μM for human PRMT1 and 3.0 μM for yeast-Hmt1p. It achieves its PRMTs inhibitory activity by obstructing peptide-substrate binding [1].

AMI-1 free acid

🥰Excellent

Purity: 97.8%

Catalog No. T22239Cas No. 134-47-4

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$40	-	In Stock
50 mg	$56	-	In Stock
100 mg	$81	-	In Stock
500 mg	$198	-	In Stock
1 g	$293	-	In Stock
1 mL x 10 mM (in DMSO)	$29	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.8%

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	AMI-1 free acid is a potent, cell-permeable, and reversible inhibitor of protein arginine N-methyltransferases (PRMTs), with inhibitory concentration 50 (IC50) values of 8.8 μM for human PRMT1 and 3.0 μM for yeast-Hmt1p. It achieves its PRMTs inhibitory activity by obstructing peptide-substrate binding [1].
In vitro	In S180 cells and U2OS cells, AMI-1, free acid (0.6 mM, 1.2 mM, 2.4 mM; 48 hours, 72 hours, 96 hours) inhibits cell viability[3].
In vivo	AMI-1, free acid (0.5 mg; administered daily via subcutaneous injection for 7 days) reduces tumor weight in male Kunming mice with S180 cell xenografts[3].

Chemical Properties

Molecular Weight	504.49
Formula	C₂₁H₁₆N₂O₉S₂
Cas No.	134-47-4
Smiles	O=C(NC=1C=CC2=C(O)C=C(C=C2C1)S(=O)(=O)O)NC=3C=CC4=C(O)C=C(C=C4C3)S(=O)(=O)O
Relative Density.	1.31g/cm3
Color	Yellow
Appearance	Solid

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (118.93 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.96 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9822 mL	9.9110 mL	19.8220 mL	99.1100 mL
5 mM	0.3964 mL	1.9822 mL	3.9644 mL	19.8220 mL
10 mM	0.1982 mL	0.9911 mL	1.9822 mL	9.9110 mL
20 mM	0.0991 mL	0.4955 mL	0.9911 mL	4.9555 mL
50 mM	0.0396 mL	0.1982 mL	0.3964 mL	1.9822 mL
100 mM	0.0198 mL	0.0991 mL	0.1982 mL	0.9911 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc