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AEBSF hydrochloride

Catalog No. T6370   CAS 30827-99-7
Synonyms: AEBSF HCl, Pefabloc SC

AEBSF hydrochloride (Pefabloc SC) is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, thrombin, plasmin, and trypsin.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
AEBSF hydrochloride Chemical Structure
AEBSF hydrochloride, CAS 30827-99-7
Pack Size Availability Price/USD Quantity
25 mg In stock $ 30.00
50 mg In stock $ 43.00
100 mg In stock $ 51.00
200 mg In stock $ 92.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 99.61%
Purity: 99.5%
Purity: 99.45%
Purity: 99%
Purity: 98.92%
Purity: 98%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description AEBSF hydrochloride (Pefabloc SC) is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, thrombin, plasmin, and trypsin.
In vitro In a mouse model of cockroach allergen-induced asthma, AEBSF was able to mitigate respiratory reactions and potential inflammation. Intraperitoneal injection of 76.8 mg/kg AEBSF daily in mice lethally inoculated with Toxoplasma gondii extended their survival period.
In vivo AEBSF is a serine protease inhibitor that suppresses the lysis of leukemia cells by macrophages without inhibiting the secretion of TNF-α and IL-1β by these cells. In five different human cell lines, AEBSF inhibits the formation of amyloid-beta (Aβ) by blocking the secretion of both neural and non-neural β-secretase products. Additionally, AEBSF disrupts embryonic growth within the endometrium and modifies protein secretion mechanisms to prevent the adhesion of HeLa cells in human umbilical vein endothelial cells.
Cell Research The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm.
Synonyms AEBSF HCl, Pefabloc SC
Molecular Weight 239.69
Formula C8H11ClFNO2S
CAS No. 30827-99-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 24 mg/mL (100 mM)

DMSO: 24 mg/mL (100 mM)

TargetMolReferences and Literature

1. Citron M, et al. Neuron. 1996, 17(1), 171-179. 2. Nakabo Y, et al. J Leukoc Biol. 1996, 60(3), 328-336. 3. Buitrago-Rey R. et al. J Antimicrob Chemother. 2002, 49(5), 871-874. 4. Jiang YH, et al. Contraception. 2011, 84(6), 642-648. 5. Saw S, et al. Inflammation. 2014. DOI 10.12007/s10753-2014-19976-0. 6. Jiang T Y, Feng X F, Fang Z, et al. PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer[J]. Cancer Letters. 2020 7. Jiang T Y, Pan Y F, Wan Z H, et al. PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma[J]. Science Translational Medicine. 2020, 12(562).

TargetMolCitations

1. Jiang T Y, Pan Y F, Wan Z H, et al. PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. Science Translational Medicine. 2020, 12(562). 2. Jiang T Y, Feng X F, Fang Z, et al. PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer. Cancer Letters. 2021, 501: 187-199.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library Anti-Aging Compound Library Fluorochemical Library Covalent Inhibitor Library Bioactive Compounds Library Max NO PAINS Compound Library Bioactive Compound Library Anti-Metabolism Disease Compound Library Anti-Infection Compound Library Target-Focused Phenotypic Screening Library

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Keywords

AEBSF hydrochloride 30827-99-7 Microbiology/Virology Proteases/Proteasome Thrombin Serine Protease Influenza Virus AEBSF Ser/Thr Protease Threonine proteases inhibit AEBSF Hydrochloride Serine endopeptidases Serine proteases Inhibitor AEBSF HCl Pefabloc SC inhibitor

 

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