Powder: -20°C for 3 years | In solvent: -80°C for 1 year
AEBSF hydrochloride (Pefabloc SC) is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, thrombin, plasmin, and trypsin.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
25 mg | In stock | $ 30.00 | |
50 mg | In stock | $ 43.00 | |
100 mg | In stock | $ 51.00 | |
200 mg | In stock | $ 92.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | AEBSF hydrochloride (Pefabloc SC) is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, thrombin, plasmin, and trypsin. |
In vitro | In a mouse model of cockroach allergen-induced asthma, AEBSF was able to mitigate respiratory reactions and potential inflammation. Intraperitoneal injection of 76.8 mg/kg AEBSF daily in mice lethally inoculated with Toxoplasma gondii extended their survival period. |
In vivo | AEBSF is a serine protease inhibitor that suppresses the lysis of leukemia cells by macrophages without inhibiting the secretion of TNF-α and IL-1β by these cells. In five different human cell lines, AEBSF inhibits the formation of amyloid-beta (Aβ) by blocking the secretion of both neural and non-neural β-secretase products. Additionally, AEBSF disrupts embryonic growth within the endometrium and modifies protein secretion mechanisms to prevent the adhesion of HeLa cells in human umbilical vein endothelial cells. |
Cell Research | The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm. |
Synonyms | AEBSF HCl, Pefabloc SC |
Molecular Weight | 239.69 |
Formula | C8H11ClFNO2S |
CAS No. | 30827-99-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 24 mg/mL (100 mM)
DMSO: 24 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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AEBSF hydrochloride 30827-99-7 Microbiology/Virology Proteases/Proteasome Thrombin Serine Protease Influenza Virus AEBSF Ser/Thr Protease Threonine proteases inhibit AEBSF Hydrochloride Serine endopeptidases Serine proteases Inhibitor AEBSF HCl Pefabloc SC inhibitor