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3,4-Dihydroxybenzylamine hydrobromide

Catalog No. T10104   CAS 16290-26-9
Synonyms: NSC 263475 hydrobromide

3,4-Dihydroxybenzylamine hydrobromide (NSC-263475 hydrobromide) inhibits DNA polymerase activity in melanoma cells and displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity.

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3,4-Dihydroxybenzylamine hydrobromide Chemical Structure
3,4-Dihydroxybenzylamine hydrobromide, CAS 16290-26-9
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100 mg In stock $ 41.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 97.28%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description 3,4-Dihydroxybenzylamine hydrobromide (NSC-263475 hydrobromide) inhibits DNA polymerase activity in melanoma cells and displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity.
In vitro 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) inhibits the growth of all melanoma cell lines (IC50 = 100 μM) and the SK-MELB cell line (IC50 = 122 μM). The IC50 values are 30, 84,90, and 68 μM for Human SK-MEL-30, SK-MEL-2, SK-MEL-3,RPMI-7951 cells, respectively. 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) cytotoxicity in SK-MEL-2 and SK-MEL-28 cell lines can be enhanced by application of buthionine sulfoximine (BSO). BSO treatment sensitizes marginally pigmented cells to the effects of 3,4-DHBA, and the presence of decreses IC50 values of 3,4-DHBA by 127% in the SK-MEL-28 cell line and by 148% in the SK-MEL-2 cell line. 3,4-Dihydroxybenzylamine hydrobromide exhibits IC50values of 10, 25, 67, and 184 μM for S91A, S91B, L1210, and SCC-25 cells, respectively[1].
In vivo 3,4-Dihydroxybenzylamine hydrobromide (200, 400, 600 and 800 mg/kg; i.p.; 21 days) has different effects at different doses, the median life-span are 17, 24.5, 26, 29 and 25 days respectively. 3,4-Dihydroxybenzylamine hydrobromide (1000 mg/kg; i.p.; 7 days) has the least toxic effect in non-tumor-bearing B6D2F1 mice,and mice is tolerated at this dose[2].
Cell Research Cell Line: Human and Murine Melanoma Cell Lines; Non-Melanoma Cell Lines Concentration: 1 μM-10 mM Incubation Time: 48 hours [1]
Animal Research Animal Model: C57BL/6 mice with B6D2F1 cells Dosage: 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg Administration: Intraperitoneal injection [2]
Synonyms NSC 263475 hydrobromide
Molecular Weight 220.06
Formula C7H10BrNO2
CAS No. 16290-26-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 80 mg/mL (363.54 mM), sonification is recommended.

TargetMolReferences and Literature

1. Prezioso JA1,et al.Effects of tyrosinase activity on the cytotoxicity of 3,4-dihydroxybenzylamine and buthionine sulfoximine in human melanoma cells.Pigment Cell Res. 1990 Mar-Apr;3(2):49-54. 2. Mlchael M. Wick,et al. 3,4-Dihydroxybenzylamine: Antitumor Activity Against A Dopamine Analog B16 Melanoma. 3. FitzGerald GB, et al. 3,4-Dihydroxybenzylamine: an improved dopamine analog cytotoxic for melanoma cells in part through oxidation products inhibitory to dna polymerase.J Invest Dermatol. 1983 Feb;80(2):119-23.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compounds Library Max DNA Damage & Repair Compound Library Inhibitor Library Bioactive Compound Library Anti-Cancer Compound Library

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Keywords

3,4-Dihydroxybenzylamine hydrobromide 16290-26-9 Cell Cycle/Checkpoint DNA Damage/DNA Repair Others DNA/RNA Synthesis NSC-263475 Hydrobromide Inhibitor NSC 263475 hydrobromide DNA polymerase Tumor NSC263475 melanoma NSC-263475 3,4-Dihydroxybenzylamine inhibit 3,4Dihydroxybenzylamine hydrobromide tyrosinase NSC263475 Hydrobromide NSC 263475 Hydrobromide NSC 263475 3,4-Dihydroxybenzylamine Hydrobromide 3,4 Dihydroxybenzylamine hydrobromide inhibitor

 

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