Powder: -20°C for 3 years | In solvent: -80°C for 1 year
3,4-Dihydroxybenzylamine hydrobromide (NSC-263475 hydrobromide) inhibits DNA polymerase activity in melanoma cells and displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 mg | In stock | $ 41.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | 3,4-Dihydroxybenzylamine hydrobromide (NSC-263475 hydrobromide) inhibits DNA polymerase activity in melanoma cells and displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity. |
In vitro | 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) inhibits the growth of all melanoma cell lines (IC50 = 100 μM) and the SK-MELB cell line (IC50 = 122 μM). The IC50 values are 30, 84,90, and 68 μM for Human SK-MEL-30, SK-MEL-2, SK-MEL-3,RPMI-7951 cells, respectively. 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) cytotoxicity in SK-MEL-2 and SK-MEL-28 cell lines can be enhanced by application of buthionine sulfoximine (BSO). BSO treatment sensitizes marginally pigmented cells to the effects of 3,4-DHBA, and the presence of decreses IC50 values of 3,4-DHBA by 127% in the SK-MEL-28 cell line and by 148% in the SK-MEL-2 cell line. 3,4-Dihydroxybenzylamine hydrobromide exhibits IC50values of 10, 25, 67, and 184 μM for S91A, S91B, L1210, and SCC-25 cells, respectively[1]. |
In vivo | 3,4-Dihydroxybenzylamine hydrobromide (200, 400, 600 and 800 mg/kg; i.p.; 21 days) has different effects at different doses, the median life-span are 17, 24.5, 26, 29 and 25 days respectively. 3,4-Dihydroxybenzylamine hydrobromide (1000 mg/kg; i.p.; 7 days) has the least toxic effect in non-tumor-bearing B6D2F1 mice,and mice is tolerated at this dose[2]. |
Cell Research | Cell Line: Human and Murine Melanoma Cell Lines; Non-Melanoma Cell Lines Concentration: 1 μM-10 mM Incubation Time: 48 hours [1] |
Animal Research | Animal Model: C57BL/6 mice with B6D2F1 cells Dosage: 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg Administration: Intraperitoneal injection [2] |
Synonyms | NSC 263475 hydrobromide |
Molecular Weight | 220.06 |
Formula | C7H10BrNO2 |
CAS No. | 16290-26-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 80 mg/mL (363.54 mM), sonification is recommended.
You can also refer to dose conversion for different animals. More
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3,4-Dihydroxybenzylamine hydrobromide 16290-26-9 Cell Cycle/Checkpoint DNA Damage/DNA Repair Others DNA/RNA Synthesis NSC-263475 Hydrobromide Inhibitor NSC 263475 hydrobromide DNA polymerase Tumor NSC263475 melanoma NSC-263475 3,4-Dihydroxybenzylamine inhibit 3,4Dihydroxybenzylamine hydrobromide tyrosinase NSC263475 Hydrobromide NSC 263475 Hydrobromide NSC 263475 3,4-Dihydroxybenzylamine Hydrobromide 3,4 Dihydroxybenzylamine hydrobromide inhibitor